缺氧诱导有丝分裂因子收缩大鼠主动脉壁血管平滑肌细胞的途径探讨  

Mechanism of hypoxia-induced mitogenie factor underlying contraction of vascular smooth muscle cells in rat aorta wall

作　　者：张洪强 段淑香 杨永曜[3] 徐庆国 Zhang Hongqiang;Duan Shuxiang;Yang Yongyao;Xu Qingguo(The Second Hospital of Shandong University,Jinan 250033,Shandong Province,China)

机构地区：[1]山东大学第二医院急诊医学中心,济南250033 [2]山东省立第三医院心内科 [3]贵州省人民医院心内科

出　　处：《中华老年心脑血管病杂志》2019年第11期1196-1198,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81660080);国家临床重点专科建设[国卫办医函(2013)544号];贵州省科技计划项目[黔科合平台人才(2017)5405]

摘　　要：目的 探讨发现于炎症区域1(FIZZ1)对大鼠主动脉平滑肌细胞(RASMC)的收缩作用机制。方法 采用贴壁法培养RASMC,随机分为正常对照组,阳性对照组(血管紧张素Ⅱ终浓度1×10^-7 mol/L),FIZZ1刺激组、2倍FIZZ1刺激组和4倍FIZZ1刺激组(分别用1×10^-8 mol/L、2×10^-8 mol/L和4×10^-8 mol/L FIZZ1),调钙蛋白(CaM)抑制剂组和肌球蛋白轻链激酶(MLCK)抑制剂组(分别采用FIZZ1+CaM抑制剂W-7或MLCK抑制剂ML-7),各组分别刺激RASMC 48h。用RT-PCR测定各组CaM和MLCK mRNA表达。结果 4倍FIZZ1刺激组CaM mRNA显著高于正常对照组和阳性对照组,差异有统计学意义(1.22±0.31 vs 0.28±0.06和0.60±0.10,P<0.05)、且MLCK mRNA表达水平显著高于正常对照组和阳性对照组,差异有统计学意义[(7.92±2.60)×10^-4 vs (2.01±1.08)×10^-4和(4.80±1.21)×10^-4,P<0.05]。CaM抑制剂组和MLCK抑制剂组较3个FIZZ1刺激组CaM和MLCK mRNA表达明显降低,差异有统计学意义(P<0.05)。结论FIZZ1可能通过Ca2+-CaMMLCK途径引起RASMC收缩。Objective To study the contraction mechanism of rat aortil smooth muscle cells(RASMC)in rat aorta wall by found in inflammatory zone 1(FIZZ1).Methods The adherently cultured RASMC were divided into normal control group,positive control group(1×10^-7 mol/L AngⅡ),FIZZ1 stimulation group(1×10^-8 mol/L FIZZ1),2-fold FIZZ1 stimulation group(2×10^-8 mol/L FIZZ1),4-fold FIZZ1 stimulation group(4×10^-8 mol/L FIZZ1),CaM inhibitor group and MLCK inhibitor group.The expressions of CaM and MLCK mRNA in different groups were detected by RT-PCR after the RASMC were stimulated for 48 h.Results The expression levels of CaM and MLCK mRNA were significantly higher in 4-fold FIZZ1 stimulation group than in normal control group and positive control group(1.22±0.31 vs 0.28±0.06 and 0.60±0.10,P<0.05;7.92×10^-4±2.60×10^-4 vs 2.01×10^-4±1.08×10^-4 and 4.80×10^-4±1.21×10^-4,P<0.05)while those of CaM and MLCK mRNA were significantly lower in CaM inhibitor group and MLCK inhibitor group than in FIZZ1 stimulation group,2-fold FIZZ1 stimulation group and 4-fold FIZZ1 stimulation group(P<0.05).Conclusion FIZZ1 can induce the contraction of RASMC in rat aortic wall through the Ca2+-CaM-MLCK pathway.

关 键 词：肌细胞 平滑肌 缺氧 有丝分裂 高血压 肺性 肌球蛋白轻链激酶 主动脉 

分 类 号：R36[医药卫生—病理学]