循环肿瘤DNA的临床应用研究进展  

Progress in clinical application of circulating tumor DNA

作　　者：程筱雯[1] CHENG Xiaowen(Department of Clinical Laboratory,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui,China,230022)

机构地区：[1]安徽医科大学第一附属医院检验科

出　　处：《分子诊断与治疗杂志》2019年第6期539-544,共6页Journal of Molecular Diagnostics and Therapy

基　　金：国家青年科学基金(81302150);安徽省级质量工程(2014jyxm761)

摘　　要：近年来,基因组的深入研究和各种分子生物学新技术的快速发展使得循环肿瘤DNA(ctDNA)的检测分析正在从研究领域向临床应用过渡。大量研究结果表明ctDNA在肿瘤的预测诊断、病情监测和预后判断等多方面显示出广阔的发展前景。ctDNA是肿瘤患者血液中存在的异常的细胞游离DNA(cfDNA),尽管cfDNA的生物学机制还不甚清楚,但ctDNA分析已经开始在临床上应用,这有助于推进肿瘤精准医疗的发展。本文主要从ctDNA的检测方法、ctDNA在肿瘤精准医疗中的应用及ctDNA检测分析面临的挑战等方面进行了综述,以期进一步探讨ctDNA切实可行的临床应用方向。With the rapid development of various new molecular methods and the improvements in genomic,the analysis of circulating tumor DNA(ctDNA)is gradually shifting from basic research to clinical application.Numerous studies have shown that ctDNA has a broad prospect for prediction,monitoring and prognostication of tumors.ctDNA is an abnormal cell free DNA(cfDNA)present in the blood of tumor patients.Although the biological mechanism of cfDNA is not clear,ctDNA analysis has begun to be applied in clinical practice,which is helpful to promote the development of accurate tumor treatment.In this paper,the detection methods of ctDNA,the application of ctDNA in cancer precision medicine and the challenges of ctDNA detection and analysis are reviewed,in order to further explore the practical clinical application of ctDNA.

关 键 词：循环肿瘤DNA 定量PCR 下一代测序 肿瘤精准医疗 

分 类 号：R73[医药卫生—肿瘤]