BoneClear: whole-tissue immunolabeling of the intact mouse bones for 3D imaging of neural anatomy and pathology  

作　　者：Qi Wang Kaili Liu Lu Yang Huanhuan Wang Jing Yang 

机构地区：[1]State Key Laboratory of Membrane Biology,Peking University,beijing 100871,China [2]Center for Life Sciences,Peking University,Beijing 100871,China [3]Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China [4]School of Life Sciences,Peking University,Beijing 100871,China [5]IDG/McGovern Institute for Brain Research,Peking University,Beijing 100871,China

出　　处：《Cell Research》2019年第10期870-872,共3页细胞研究（英文版）

基　　金：the State Key Laboratory of Membrane Biology,the IDG/McGovern Institute for Brain Research;the Center for Life Sciences and the School of Life Sciences at Peking University;by the National Natural Science Foundation of China(31522024 and 31771111).

摘　　要：Dear Editor,Current approaches to analyze cellular structures in the bone tissues have depended mainly on tissue sections.For instance,a recent study visualized different cellular populations in the 300-um sections of the mouse femurs1.Similarly,the distribution of hematopoietic stem cells could be examined in the half-bone sections2.Notably,such conventional methods obscure specific cellular structures,e.g.,neural innervations,that would be better observed with the intact,unsectioned bones.Moreover,histological sectioning could be challenging for many bone tissues,e.g.,vertebral column,skull and limbs.

关 键 词：ANATOMY NEURAL PATHOLOGY 

分 类 号：R73[医药卫生—肿瘤]