Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome  

作　　者：ZHANG Jun-qin WEI Yan-ming HUANG Kun SUN Xiao-mei ZOU Zhong JIN Mei-lin 

机构地区：[1]State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,P.R.China [2]College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,P.R.China [3]Key Laboratory of Development of Veterinary Diagnostic Products,Ministry of Agriculture,Wuhan 430070,P.R.China

出　　处：《Journal of Integrative Agriculture》2019年第11期2598-2604,共7页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (2016YFD0500801)

摘　　要：Hepatitis-hydropericardium syndrome(HHS)is an infectious disease caused by fowl adenovirus serotype 4(FAdV-4).Several structural and non-structural proteins of FAdV-4 have been expressed in Escherichia coli and baculovirus expression system to develop candidate subunit vaccines.However,the protective efficiency of baculovirus-expressed penton base protein has not been assessed.In this study,two recombinant capsid proteins,penton base and fiber-2,were constructed.And then,penton base and fiber-2 were administrated alone or together to specific pathogen-free(SPF)chickens at 14 days of life and boosted at 28 days of life.At 42 days of life,the immunized groups and the control group were challenged with FAdV-4 virulent strain.Results show that inoculating penton base or penton base+fiber-2 provided 100%protection to the chickens.All groups vaccinated with the recombinant protein produced detectable antibodies and showed no apparent lesions.Thus,baculovirus-expressed penton base protein is a promising candidate subunit vaccine.Hepatitis-hydropericardium syndrome(HHS) is an infectious disease caused by fowl adenovirus serotype 4(FAdV-4). Several structural and non-structural proteins of FAdV-4 have been expressed in Escherichia coli and baculovirus expression system to develop candidate subunit vaccines. However, the protective efficiency of baculovirus-expressed penton base protein has not been assessed. In this study, two recombinant capsid proteins, penton base and fiber-2, were constructed. And then, penton base and fiber-2 were administrated alone or together to specific pathogen-free(SPF) chickens at 14 days of life and boosted at 28 days of life. At 42 days of life, the immunized groups and the control group were challenged with FAdV-4 virulent strain. Results show that inoculating penton base or penton base+fiber-2 provided 100% protection to the chickens. All groups vaccinated with the recombinant protein produced detectable antibodies and showed no apparent lesions. Thus, baculovirus-expressed penton base protein is a promising candidate subunit vaccine.

关 键 词：FOWL adenovirus SEROTYPE 4 hepatitis-hydropericardium SYNDROME PENTON base subunit vaccine BACULOVIRUS expression system 

分 类 号：R73[医药卫生—肿瘤]