植物油中维生素E异构体在BEH C18和CSH Fluoro-Phenyl固定相上的保留特征及含量差异  

Retention Behavior and Content Differences of Vitamin E Isomers in Vegetable Oil on BEH C18 and CSH Fluoro-Phenyl Stationary Phases

作　　者：谢跃杰 王锴 勾瑶 任贵礼 王波 赵茹玉 王强 XIE Yue-jie;WANG Kai;GOU Yao;REN Gui-li;WANG Bo;ZHAO Ru-yu;WANG Qiang(College of Biological and Chemical Engineering,Chongqing University of Education,Chongqing 400067,China;Cooperative Innovation Center of Lipid Resources and Children’s Daily Chemicals,Chongqing University of Education,Chongqing 400067,China;Chongqing Jiangyuan Olive Company Limited,Chongqing 401587,China;Integrated Technology Central,Lanzhou Customs,Lanzhou 730000,China)

机构地区：[1]重庆第二师范学院生物与化学工程学院,重庆400067 [2]重庆第二师范学院脂质资源与儿童日化品协同创新中心,重庆400067 [3]重庆江源油橄榄开发有限公司,重庆401587 [4]兰州海关综合技术中心,甘肃兰州730000

出　　处：《食品工业科技》2019年第22期234-241,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(31871728);重庆市社会事业与民生保障科技创新专项(cstc2017shms-xdny80081);重庆市创新创业示范团队支持计划(201618793);重庆市基础研究与前沿探索项目(cstc2018jcyjAX0824);兰州海关科技计划项目(2017GK005)

摘　　要：同分异构体的鉴别一直是食品掺伪或筛查的热点问题。本文通过BEH C18和CSH Fluoro-Phenyl两种固定相,基于RP-UPLC技术,研究VE同分异构体在不同的色谱柱温度(30、35、40、45、50℃)及不同的有机相(甲醇和乙腈)的保留特征,通过分离度、拖尾因子及容量因子为指标对目标物保留特征进行评价,并在最优的色谱条件下对不同植物油样品中的VE进行了含量检测。结果表明,CSH Fluoro-Phenyl为固定相,甲醇为有机相时,除β-VE和γ-VE可进行部分的分离,具有明显的选择性差异外,其余固定相及有机相均未有明显的选择性差异。样品经乙醇提取,BEH C18为固定相,柱温30℃,进样量为1.0μL,VE异构体在0.002~20 mg/L的范围内具有良好的线性,相关系数r>0.9993;通过对实际样品的分析验证,本研究建立的方法具高效快速、操作简单、实验成本低等优点,可满足植物油中VE的快速、准确、高通量的检测。The identification of isomers is a hot issue in food adulteration or screening.The retention behaviors of VE isomers at different column temperatures(30,35,40,45,50℃)and different organic modifiers(methanol and acetonitrile)were studied by using BEH C18 and CSH Fluoro-Phenyl stationary phases,the retention behaviors of the target compounds were evaluated by resolution,trailing factor and capacity factor,and the contents of VE in different vegetable oil samples were determined under the optimal chromatographic conditions.The results showed that when CSH Fluovo-Phenyl was used as stationary phase and methanol as organic phase,except forβ-VE andγ-VE could be partially separated,the other stationary phase and chromatographic conditions had no significant difference in selectivity.Moreover,the sample was extracted by ethanol,BEH C18 as stationary phase,the column temperature was 30℃,the injection volume was 1.0μL,the linear range of VE isomer were 0.002~20 mg/L,the correlation coefficient was r>0.9993.Through the analysis of the actual samples,the method established in this study has the advantages of high efficiency,speed and low experimental cost,which could meet the requirements of rapid,accurate and high throughput detection of VE in vegetable oils.

关 键 词：保留特征 植物油 维生素E 异构体 固定相 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]