高黏度壳聚糖回收鱼糜漂洗液中蛋白质的研究  

Research on Recovery of Protein from Surimi Rinse by High Viscosity Chitosan

作　　者：靳挺[1] 沈科丞 施亚芳 武玉学[1] Jin Ting;Shen Kecheng;Shi Yafang;Wu Yuxue(College of Biochemical Engineering,Ningbo Institute of Technology,Zhejiang University,Ningbo 315100,Zhejiang)

机构地区：[1]浙江大学宁波理工学院生物与化学工程学院

出　　处：《中国食品学报》2019年第11期165-169,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：宁波市自然科学基金项目

摘　　要：本文首次利用高黏度壳聚糖(黏度>400 m Pa·s)回收鱼糜漂洗液中的蛋白质,探讨其影响因素,以获得优化的回收工艺条件。通过单因素试验研究了温度、p H、作用时间、壳聚糖浓度对蛋白质回收率的影响,并在此基础上做正交试验,得出优化的试验条件。采用双缩脲法测定蛋白质浓度,重铬酸钾法测定COD。试验结果表明:在优化的试验条件下:pH 6.5,温度20℃,作用时间90 min,壳聚糖质量浓度1.67 mg/mL,高黏度壳聚糖对鱼糜漂洗液中蛋白质回收率达43.68%,COD去除率为22.73%。This is the first reported protein recovered from surimi rinse by high viscosity chitosan(viscosity>400 mPa·s).In order to obtain the optimal process conditions of the protein recovery,the factors influencing the protein recovery of high viscosity chitosan were studied.The factors such as temperature,pH,time,concentration of chitosan were researched by single factor experiment and the optimum conditions were obtained by the orthogonal experiments.The concentration of protein and COD were determined by using biuret method and potassium dichromate method,respectively.The optimal conditions for protein recovery were as following:pH 6.5,20℃,90 min,concentration of chitosan 1.67 mg/mL.Under the optimum conditions,the recovery rate of protein was up to 43.68%and the removal rate of COD was 22.73%.

关 键 词：高黏度壳聚糖 鱼糜漂洗液 回收 蛋白质 

分 类 号：TS2[轻工技术与工程—食品科学与工程]