食用油中苯并(α)芘含量测定的不确定度评估  

Uncertainty evaluation of the determination of benzo (α) pyrene in edible oils

作　　者：李雪[1,2,3] 张玉 王君虹[1,3] 白丽萍 朱作艺 刘军[4] 朱申龙[3] 沈国新 王伟 LI Xue;ZHANG Yu;WANG Jun-hong;BAI Li-ping;ZHU Zuo-yi;LIU Jun;ZHU Shen-long;SHEN Guo-xin;WANG Wei(Institute of Quality and Standards for Agricultural Products,Zhejiang Academy of Agricultural Sciences,Hangzhou Zhejiang 310021;Key Laboratory of Agricultural Products Information Traceability,Ministry of Agriculture and Rural Affairs,Hangzhou Zhejiang 310021;International joint laboratory of quality and nutrition of woody oils,Hangzhou Zhejiang 310021;Hangzhou Entry-Exit Inspection and Quarantine Bureau,Hangzhou Zhejiang 310012)

机构地区：[1]浙江省农业科学院农产品质量标准研究所,浙江杭州310021 [2]农业农村部农产品信息溯源重点实验室,浙江杭州310021 [3]木本油料品质营养国际联合实验室,浙江杭州310021 [4]杭州出入境检验检疫局,浙江杭州310012

出　　处：《粮油食品科技》2019年第6期102-108,共7页Science and Technology of Cereals,Oils and Foods

基　　金：省部共建国家重点实验室自主设计课题(ZZ1809);浙江省农业科学院B类学科建设项目;浙江省农业科学院国际合作项目(19LL07);浙江检验检疫局一般科研计划项目(2017-ZKZ-02)

摘　　要：通过对分子印迹柱净化法结合高效液相色谱法测定大豆油中苯并(α)芘含量的全过程逐层分析,进行测定结果不确定度的评估。应用计量学不确定度传递模型,对不确定度分量进行量化及合成,并计算其扩展不确定度。结果表明,影响不确定度的主要因素为标准曲线拟合、添加回收率、样品称量及标准工作溶液配制。当大豆油中苯并(α)芘含量为8.735μg/kg时,在95%的置信区间下,其扩展不确定度为0.794μg/kg(k=2)。评定结果表明,应用分子印迹柱净化法结合高效液相色谱法对食用油中苯并(α)芘的测定,可通过严格控制校准曲线拟合过程、添加回收过程、样品称量及标准工作液配制过程从而减小不确定度。Benzo(α)pyrene in soybean oil was determined by molecular imprinting column purification combined with high performance liquid chromatography(MIC-HPLC).The whole process was analyzed to evaluate the uncertainty of the analytical results.Meanwhile,the uncertainty components were quantified and synthesized by a metrological model of uncertainty.Furthermore,the extended uncertainty of the measurement results was calculated.The results showed that the main factors which affected the uncertainty were the calibration curve,recovery,sample weighting and working solution preparation.When the content of benzo(α)pyrene in soybean oil was 8.735μg/kg,at 95%confidence interval,the expanded uncertainty was 0.794μg/kg(k=2).The uncertainty of benzo(α)pyrene in edible oil determined by MIC-HPLC can be reduced by strictly control the processes of the curve fitting,recovery,sample weighting and working solution preparation.

关 键 词：食用油 苯并(α)芘 不确定度评估 高效液相色谱法 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]