枯草芽孢杆菌发酵制备玉米肽-葡萄糖螯合物的条件优化  

Optimization of Fermentation Conditions of Corn Peptide-Glucose Chelates by Bacillus subtilis

作　　者：刘奇[1] 张智[1] Liu Qi;Zhang Zhi(Forestry College of Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学林学院

出　　处：《中国粮油学报》2019年第11期95-99,119,共6页Journal of the Chinese Cereals and Oils Association

基　　金：黑龙江省应用技术研究与开发重大项目子课题(GA 13B201)

摘　　要：以玉米肽和葡萄糖为底物,利用枯草芽孢杆菌(Bacillus subtilis)ls-45为菌种发酵,以DPPH清除率为指标,通过单因素及响应面实验优化枯草芽孢杆菌发酵制备玉米肽-葡萄糖螯合物的条件,为研究玉米肽-葡萄糖螯合物体内、体外抗氧化等功能实验提供数据支撑。采用四因素三水平响应面实验确定最佳接种量、pH值、发酵温度及发酵时间。结果表明:利用枯草芽孢杆菌发酵制备玉米肽-葡萄糖螯合物的最佳工艺条件为接种量10%,发酵时间46 h,pH 6,发酵温度37℃,此时DPPH清除率为90.82%。Using corn peptide and glucose as substrate, Bacillus subtilis ls-45 as strain to ferment, using DPPH clearance as the index, and optimizing the condition of preparing the corn peptide-glucose complex by single factor and response surface experiment. In ord to study that in vivo and in vitro anti-oxidation and other functional experiments of the corn peptide-glucose compound. The optimal inoculum size, pH value, fermentation temperature and fermentation time were determined by four-factor three-level response surface experiment. The results showed that the optimal process conditions for preparing the corn peptide-glucose complex by Bacillus subtilis were as follows:inoculum size 10%, the fermentation time was 46 h, pH 6, fermentation temperature was 37 ℃, DPPH clearance was 90.82%.

关 键 词：玉米肽-葡萄糖螯合物 枯草芽孢杆菌 发酵 条件优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]