基于荧光共振能量转移构建关-开型复合荧光探针快速检测沙门氏菌  

Rapid Detection of Salmonella via Off-on Composite Fluorescent Probe Based on Fluorescence Resonance Energy Transfer

作　　者：崔雯雯 徐琳琳[1] 史艳宇 董娜 陈萍[1] CUI Wen-Wen;XU Lin-Lin;SHI Yan-Yu;DONG Na;CHEN Ping(Food Science and Engineering College,Jilin Agriculutral University,Changchun 130118,China;Jilin Food Inspection Institute,Changchun 130103,China)

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118 [2]吉林省食品检验所,长春130103

出　　处：《分析化学》2019年第12期1973-1980,共8页Chinese Journal of Analytical Chemistry

基　　金：吉林省省级产业创新专项资金项目(No.2018C045-2)资助~~

摘　　要：将经硅烷化以及氨基化修饰的功能化磁珠与沙门氏菌特异性抗体进行偶联,制备功能化免疫磁珠,通过近红外光谱、荧光光谱、紫外光谱等技术对其进行结构和活性表征。将功能化磁珠、抗体和量子点(QDs)进行有序组装,构建了集磁性富集分离和检测于一体的磁珠-抗体-QDs复合荧光纳米探针。基于此复合荧光纳米探针中的QDs与金纳米粒子(AuNPs)间的荧光共振能量转移(FRET)效应,建立了快速检测沙门氏菌的"关-开"型荧光分析方法。结果表明,复合荧光纳米探针的荧光恢复度与菌浓度的对数值呈良好的线性关系,线性回归方程为y=103.5x+121.4(R^2=0.9956),检出限为10^2 CFU/mL(S/N=3),检测时间少于2 h。本方法特异性好,检出限低,灵敏度高,可满足食品中沙门氏菌快速检测的需求。Immunomagnetic beads were prepared by coupling the functional magnetic beads modified by silanization and amination with Salmonella specific antibodies. The properties and biological activities of the beads were characterized by near infrared spectroscopy, fluorescence spectroscopy and absorption spectroscopy. Aminated magnetic beads, antibodies and quantum dots(QDs) were assembled in an orderly manner to construct a magnetic-bead-antibody-QDs composite fluorescent nanoprobe with magnetic collection and separation abilities for rapid detection of Salmonella. off-on fluorescence was realized through the fluorescence resonance energy transfer(FRET) effect between the QDs and gold nanoparticles(AuNPs), and an off-on fluorescent probe analytical method for rapidly detecting Salmonella was established. The results showed that the degree of fluorescence recovery of the composite fluorescent nanoprobe showed a good linear relationship with logarithm of bacterial concentration, and the linear regression equation was y=103.5x+121.4(R^2=0.9956). Moreover, the detection limit of the probe was 10^2 CFU/mL, and its detection time was less than 2 h. The proposed method features good specificity, low detection limit and high sensitivity,and can be used to rapidly detect Salmonella in food.

关 键 词：复合荧光纳米探针 荧光共振能量转移 快速检测 沙门氏菌 

分 类 号：R28[医药卫生—中药学]