利用不确定度比对乳粉中叶酸含量的快速检测方法  

Comparison of rapid detection methods of folic acid in milk powder by uncertainty

作　　者：解鑫[1] 喻东威[1] 马文丽[1] 赵媛[1] 刘萍萍[1] 梁春梅[1] 宋晓东[1] XIE Xin;YU Dong-Wei;MA Wen-Li;ZHAO Yuan;LIU Ping-Ping;LIANG Chun-Mei;SONG Xiao-Dong(Inner Mongolia MengNiu Dairy Products Co.,Ltd,Hohhot 011517,China)

机构地区：[1]内蒙古蒙牛乳业(集团)股份有限公司中心实验室

出　　处：《食品安全质量检测学报》2019年第21期7290-7294,共5页Journal of Food Safety and Quality

基　　金：2018年国家重点研发计划重点专项(2018YFC1604202)~~

摘　　要：目的 利用不确定度比对乳粉中叶酸含量的快速检测方法 .方法分别用进口和国产的微生物法和酶联免疫法的试剂盒对代表性乳粉样品进行3 d 3平行检测,对检测数据做正态分布分析及不确定度计算.结果 各方法检测结果正态分布检验值(anderson-darling,AD)均小于临界值0.725(95%置信区间),服从正态分布;微生物快速检测方法不确定度范围为13.3%~27.4%;酶联免疫方法不确定度范围为9.6%~31.3%.结论 微生物试剂盒快速检测方法,具有稳定性较好,品牌差异较小的特点,适合质控品的长期监测,质控图的建立.酶联免疫检测方法,具有简单、快速的特点,适合样品中叶酸的快速检测.Objective To compare the rapid detection methods of folic acid in milk powder by uncertainty Methods Representative milk powder samples were tested for 3 d 3 in parallel using imported and domestic microbiological and enzyme-linked immunosorbent assay kits. Normal distribution analysis and uncertainty calculation for test data, and normal distribution analysis and uncertainty calculation were performed. Results The normal distribution test value(anderson-darling, AD) of each method was less than the critical value of 0.725(95% confidence interval), which indicated the data subjected to normal distribution. The uncertainty of microbial rapid detection method ranged from 13.3% to 27.4%. The uncertainty of the immunization method ranged from 9.6% to 31.3%. Conclusion Rapid detection method of microbial kit has the characteristics of good stability and small brand difference, suitable for long-term monitoring of quality control products and establishment of quality control chart. The enzyme-linked immunosorbent assay has the characteristics of simple and rapid, and is suitable for rapid detection of folic acid in samples.

关 键 词：乳粉 叶酸 微生物法 酶联免疫法 不确定度 

分 类 号：TS2[轻工技术与工程—食品科学与工程]