Mitochondrial metabolomic profiling for elucidating the alleviating potential of Polygonatum kingianum against high-fat diet-induced nonalcoholic fatty liver disease  

作　　者：Xing-Xin Yang Jia-Di Wei Jian-Kang Mu Xin Liu Feng-Jiao Li Yan-Qin Li Wen Gu Jing-Ping Li Jie Yu 

机构地区：[1]College of Pharmaceutical Science,Yunnan University of Chinese Medicine,Kunming 650500,Yunnan Province,China [2]Beijing Entry-Exit Inspection and Quarantine Bureau,Beijing 100026,China

出　　处：《World Journal of Gastroenterology》2019年第43期6404-6415,共12页世界胃肠病学杂志（英文版）

基　　金：the National Natural Science Foundation of China,No.81660596;the National Natural Science Foundation of China,No.81760733;the Science and Technology Project of Yunnan China,No.2017FF117-013;the Science and Technology Project of Yunnan China,No.2016FD050;the Application and Basis Research Project of Yunnan China,No.201801CH00227

摘　　要：BACKGROUND Developing mitochondrial regulators/nutrients from natural products to remedy mitochondrial dysfunction represent attractive strategies for therapy of nonalcoholic fatty liver disease(NAFLD).Polygonatum kingianum(PK)has been traditionally used in China as a medicinal and nutritional ingredient for centuries and can alleviate high-fat diet(HFD)-induced NAFLD by promoting mitochondrial functions.To date,the underlying molecular mechanism of PK for treating mitochondrial dysfunctions and thus alleviating NAFLD remains unclear.AIM To identify the molecular mechanism behind the mitochondrial regulatory action of PK against HFD-induced NAFLD in rats.METHODS NAFLD model was induced in rats with HFD.The rats were intragastrically administered PK(4 g/kg per day)for 14 wk.Metabolites in hepatic mitochondrial samples were profiled through ultra-high performance liquid chromatography/mass spectrometry followed by multivariate statistical analysis to find the potential biomarkers and metabolic pathways.RESULTS PK significantly restored the metabolites’levels in the mitochondrial samples.Ten potential biomarkers were identified in the analyzed samples.These biomarkers are involved in riboflavin metabolism.CONCLUSION PK can alleviate HFD-induced NAFLD by regulating the riboflavin metabolism and further improving the mitochondrial functions.Thus,PK is a promising mitochondrial regulator/nutrient for alleviating NAFLD-associated diseases.BACKGROUND Developing mitochondrial regulators/nutrients from natural products to remedy mitochondrial dysfunction represent attractive strategies for therapy of nonalcoholic fatty liver disease(NAFLD). Polygonatum kingianum(PK) has been traditionally used in China as a medicinal and nutritional ingredient for centuries and can alleviate high-fat diet(HFD)-induced NAFLD by promoting mitochondrial functions. To date, the underlying molecular mechanism of PK for treating mitochondrial dysfunctions and thus alleviating NAFLD remains unclear.AIM To identify the molecular mechanism behind the mitochondrial regulatory action of PK against HFD-induced NAFLD in rats.METHODS NAFLD model was induced in rats with HFD. The rats were intragastrically administered PK(4 g/kg per day) for 14 wk. Metabolites in hepatic mitochondrial samples were profiled through ultra-high performance liquid chromatography/mass spectrometry followed by multivariate statistical analysis to find the potential biomarkers and metabolic pathways.RESULTS PK significantly restored the metabolites’ levels in the mitochondrial samples.Ten potential biomarkers were identified in the analyzed samples. These biomarkers are involved in riboflavin metabolism.CONCLUSION PK can alleviate HFD-induced NAFLD by regulating the riboflavin metabolism and further improving the mitochondrial functions. Thus, PK is a promising mitochondrial regulator/nutrient for alleviating NAFLD-associated diseases.

关 键 词：Metabolomics MITOCHONDRIA Multivariate statistical analysis Non-alcoholic FATTY liver POLYGONATUM kingianum Ultra-high performance liquid chromatography/mass SPECTROMETRY 

分 类 号：R57[医药卫生—消化系统]