UPLC法测定白芍浸膏中6种成分  

Determination of the Six Compounds in Paeoniae Radix Alba Extract by UPLC

作　　者：牛涛[1] 徐波[1] 陈红[1] 张学敏 Niu Tao;Xu Bo;Chen Hong;Zhang Xuemin(Tianjin Tasly Modern TCM Resources CO.,LTD,Tianjin 300410,China)

机构地区：[1]天津天士力现代中药资源有限公司

出　　处：《亚太传统医药》2019年第11期76-78,共3页Asia-Pacific Traditional Medicine

基　　金：天津市科技型中小企业技术创新基金(16YDLJSY00090)

摘　　要：目的:建立一种超高效液相色谱法(UPLC)同时检测白芍浸膏中芍药苷、芍药内酯苷、五没食子酰葡萄糖、没食子酸、苯甲酸、儿茶素6种成分。方法:采用ACQUITY UPLC HSS T3色谱柱,以乙腈和0.1%磷酸为流动相,梯度洗脱,流速:0.4 mL/min,检测波长:230 nm。结果:白芍浸膏中的芍药苷和芍药内酯苷等6种成分在测定范围内具有良好的线性关系(r≥0.999),精密度符合要求,平均回收率在98.5%~100.3%(n=6)。结论:该方法可以快速、准确测定白芍浸膏中的芍药苷等6种成分。Objective:To establish a UPLC method for determination of paeoniflorin、albiflorin、pentagalloylglucose、gallic acid、benzoic acid and catechin in Paeoniae Radix Alba Extract.Methods:ACQUITY HSS T3 column together with mobile phase of 0.1%phosphate acid water and acetonitrile gradient elution are used to separate the compounds.The flow rate is 0.4 mL/min.Detection wavelength was set at 230 nm.Results:Under the condition above,the six compounds are well separated,Satisfactory linearity was achieved within the investigated linear range and with fine determination coefficien(r≥0.999),the overrall recoveries are between 98.5%and 100.3%with a RSD less than 3.0%.Conclusion:The method we use is fast,easy and highly effective,it can be wildly used in the separation of flavonoids in Radix astragal extract.

关 键 词：白芍浸膏 白芍 UPLC 芍药苷 芍药内酯苷 五没食子酰葡萄糖 没食子酸 苯甲酸 儿茶素 

分 类 号：R284.2[医药卫生—中药学]