超高效液相色谱法测定食用菌中7种脂溶性维生素  

Determination of 7 fat-soluble vitamins in edible fungi by ultra-high performance liquid chromatography

作　　者：邓梦雅[1] 甘旺 岑干君 朱丽[1] 张协光[1] DENG Mengya;GAN Wang;CEN Ganjun;ZHU Li;ZHANG Xieguang(Shenzhen Academy of Metrology&Quality Inspection,National Nutritional Food Testing Center(Guangdong),Shenzhen 518131)

机构地区：[1]深圳市计量质量检测研究院国家营养食品质量监督检验中心(广东)

出　　处：《食品科技》2019年第10期341-345,共5页Food Science and Technology

基　　金：深圳市计量质量检测研究院科研项目(2018-YA19)

摘　　要：采用超高效液相色谱法同时测定食用菌中VA、VD2、α-生育酚、β-生育酚、γ-生育酚、δ-生育酚、麦角甾醇共7种脂溶性维生素的含量。样品经皂化、萃取、氮吹复溶处理后,用Phenomenex Kinetex PFP(250 mm×4.6 mm,5μm)分离,以水(A)-甲醇(B)(体积比,0~15 min,15%A^5%A;15~18min,5%A;18~20 min,5%A^15%A)为流动相,柱温25℃,流速0.8m L/min,进样量10μL,检测波长VA为325 nm,VD2为264 nm,VE为294 nm,麦角甾醇为281 nm,进入紫外检测器进行检测。7种目标物在16 min内分离出峰,在各自的质量范围内线性关系良好(r=0.9999),检出限为0.001~0.01 mg/kg,定量限为0.004~0.04 mg/kg,在3个添加水平下的平均加标回收率为82.7%~101%,相对标准偏差(RSD)为0.50%~4.66%(n=3)。该方法准确、高效、结果可靠,适用于食用菌中7种脂溶性维生素的同时测定。The content of VA, VD2, α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol and ergosterol in edible fungi was determined by ultra-high performance liquid chromatography. Phenomenex Kinetex PFP(250 mm×4.6 mm, 5 μm) was used to separate the samples with water(A)-methanol(B)(volume ratio, 0~15 min, 15%A^5%A;15~18 min, 5%A;18~20 min, 5%A^15%A) as mobile phase. The column temperature was 25 ℃, flow rate was 0.8 mL/min, sample quantity was 10 μL. The detection wavelength of VA was 325 nm, VD2 was 264 nm, VE was 294 nm, ergosterol was 281 nm, and was detected by UV detector. The peaks of the 7 targets were isolated within 16 min, and the linear relationship was good within their respective mass ranges(r=0.9999). The detection limit was 0.001~0.01 mg/kg, the quantitative limit was 0.004~0.04 mg/kg, the average standard recovery rate was 82.7%~101% at the three addition levels, and the relative standard deviation(RSD) was 0.50%~4.66%(n=3). The method is accurate, efficient and reliable. It is suitable for the simultaneous determination of 7 fat-soluble vitamins in edible fungi.

关 键 词：超高效液相色谱法 食用菌 脂溶性维生素 

分 类 号：O657.72[理学—分析化学]