Copy Number Aberrations of Multiple Genes Identified as Prognostic Markers for Extrahepatic Metastasis-free Survival of Patients with Hepatocellular Carcinoma  

作　　者：Zhong-zheng ZHU Ling-ling BAO Kun ZHAO Qing XU Jia-yi ZHU Ke-xuan Zhu Bing-ji WEN Ying-quan YE Xiao-xi WAN Liang-liang WANG Song-qin HE Wen-ming CONG 

机构地区：[1]Department of Oncology,Shanghai Tenth People fs Hospital,Tongji University School of Medicine,Shanghai 200072,China [2]Department of Oncology,No.113 Hospital of People's Liberation Army,Anhui Medical University,Ningbo 315040,China [3]Department of Education,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China [4]Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [5]Department of Burns and Plastic Surgery,General Hospital of Liaoning Provincial Armed Police Force of PLA,Shenyang 110034,China [6]Department of Pathology,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200438,China

出　　处：《Current Medical Science》2019年第5期759-765,共7页当代医学科学（英文）

基　　金：This work was supported by grants from the Medical Science and Technology Innovation Fund of PLA,Nanjing branch,China(No.14ZD07 and No.08MA023);Ningbo Natural Science Foundation Program(No.2009A610126).

摘　　要：Extrahepatic metastasis confers unfavorable patient prognosis in patients with hepatocellular carcinoma(HCC),however,reliable markers allowing prediction of extrahepatic metastasis at the time of initial diagnosis are still lacking.This study was to identify gene-level copy number aberrations(CNAs)related to extrahepatic metastasis-free survival of HCC patients,and further examine the associations between CNAs and gene expression.Array comparative genomic hybridization(aCGH)and expression array were used to analyze gene CNAs and expression levels,respectively.The associations between CNAs of a panel of 20 genes and extrahepatic metastasis-free survival were analyzed in 66 patients with follow-up period of 1.6-90.5 months.The gene expression levels between HCCs with and without gene CNA were compared in 109 patients with HCC.We observed that gains at MDM4 and BCL2L1,and losses at APC and FBXW7 were independent prognostic markers for extrahepatic metastasis-free survival of HCC patients.Integration analysis of aCGH and expression data showed that MDM4 and BCL2L1 were significantly upregulated in HCCs with gene gain,while APC and FBXW7 were significantly downregulated in HCCs with gene loss.We concluded that gene gains at MDM4 and BCL2L1,and losses at APC and FBXW7,with concordant expression changes,were associated with extrahepatic metastasis-free survival of HCC patients and have potential to act as novel prognostic markers.

关 键 词：hepatocellular carcinoma EXTRAHEPATIC metastasis-free SURVIVAL gene COPY number aberration expression 

分 类 号：R73[医药卫生—肿瘤]