Ganoderma sp. X1固态发酵改善酒糟品质  

Improvement of distillers grains quality by Ganoderma sp. X1 under solid-state fermentation

作　　者：裴久文 方勇 高学志 谢国排 房伟[1,2,3] 方泽民 PEI Jiu-wen;FANG Yong;GAO Xue-zhi;XIE Guo-pai;FANG Wei;FANG Ze-min(School of Life Sciences,Anhui University,Hefei 230601;Anhui Key Laboratory of Modern Biomanufacturing,Hefei 230601;Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis,Hefei 230601;Fuyang Livestock and Poultry Breeding Service Center,Fuyang 236012,China)

机构地区：[1]安徽大学生命科学学院,合肥230601 [2]现代生物制造安徽省重点实验室,合肥230601 [3]安徽省微生物与生物催化工程研究中心,合肥230601 [4]阜阳市畜禽养殖服务中心,阜阳236012

出　　处：《生物学杂志》2019年第6期64-68,共5页Journal of Biology

基　　金：安徽省科技重大专项项目“白酒糟转化生产高蛋白饲料及应用”(17030701059);安徽省高校自然科学研究项目“白酒糟微生物深度发酵”(KJ2017A020)

摘　　要：白酒糟含有丰富的营养成分,可开发为高蛋白饲料饲喂动物,但木质素的存在降低了动物对酒糟的利用率。为提升酒糟的利用价值,研究了固态发酵条件下真菌Ganoderma sp.X1对酒糟木质素的降解。采用正交试验,分别优化了培养基含水量、培养温度、接种量等条件。结果显示,Ganoderma sp.X1发酵白酒糟的最适发酵条件为培养基含水量75%、发酵温度28℃、接种量10%。发酵至第15天,白酒糟中木质素降解率最高达47.10%,粗蛋白由11.41%增加到17.30%。在此基础上,采用单因素试验探究麸皮的添加对Ganoderma sp.X1降解白酒糟木质素的影响。结果表明,以酒糟∶麸皮(5∶5)为固态发酵基质,发酵至第10天,木质素降解率可达46.36%,粗蛋白增加至24.80%。因此,麸皮的添加可有效缩短发酵周期,同时提高发酵产物品质。Distillers grains can be used as the feed for animals because of the rich nutrients.However,the lignin in distillers grains inhibits their utilization by animals.In this study,the conditions for lignin degradation by Ganoderma sp.X1 under solid-state fermentation were optimized,including moisture,temperature and inoculation volume of strain.The results of orthogonal experiments showed that the optimum conditions for Ganoderma sp.X1 to degrade lignin were 75%moisture content,10%inoculation volume,and at 28℃.After incubating for 15 days,the degradation rate of lignin in distillers′grains was up to 47.10%,and the crude protein increased from 11.41%to 17.30%.The effect of wheat bran on lignin degradation of distillers grains by Ganoderma sp.X1 was further studied using the single factor strategy.After fermentation for 10 days,the lignin degradation rate reached 46.36%and the crude protein increased to 24.80%when the proportion of distillers grains:wheat bran was 5∶5.These results showed that addition of wheat bran could shorten the fermentation time and improve the quality of distillers grains as the feed for animals.

关 键 词：GANODERMA sp.X1 白酒糟 木质素 

分 类 号：Q939.9[生物学—微生物学]