马铃薯酯酰基水解酶的特性研究  

Investigation on the characteristics of the activity of potato lipid acyl hydrolase

作　　者：吴乔羽 徐姗楠[2,3] 翁晴 杨丹璐 龚盛祥 张维 吴金鸿 王正武[1] WU Qiao-yu;XU Shan-nan;WENG Qin;YANG Dan-lu;GONG Sheng-xiang;ZHANG Wei;WU Jin-hong;WANG Zheng-wu(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture,Guangzhou,Guangdong 510300,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510300,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]中国水产科学研究院南海水产研究所,广东广州510300 [3]农业部南海渔业资源开发利用重点实验室,广东广州510300

出　　处：《食品与机械》2019年第11期41-46,共6页Food and Machinery

基　　金：国家自然科学基金项目（编号：31471623,21276154）;国家重点研发计划项目（编号：2016YFD0400206)

摘　　要：从马铃薯生产废水中提取分离马铃薯蛋白Patatin组分,并以对硝基苯乙酸酯为底物,研究了Patatin作为酯酰基水解酶的酶学性质。结果表明,马铃薯酯酰基水解酶在37℃时活性最高,在40~50℃的条件下具有热稳定性,70℃处理120min后酶失活;马铃薯酯酰基水解酶发挥酶活的最适pH为8.2;Fe^2+、Fe^3+、Mg^2+对马铃薯酯酰基水解酶具有活性激活作用,K^+、Ca^2+对马铃薯酯酰基水解酶活性具有抑制作用。此外,低浓度的Al^3+和Zn^2+对酯酰基水解酶的水解能力具有促进作用,高浓度的Zn^2+抑制了酯酰基水解酶的活性。In this paper,the Patatin protein was extracted from potato fruit juice,and the enzymatic properties of Patatin protein as ester acyl hydrolase were studied using p-nitrophenyl acetate as the substrate.The results showed that the lipid acyl hydrolase had the highest activity at 37 ℃,and had thermal stability at40~50℃.The enzyme was inactivated after treatment at 70 ℃for 120 min.The optimal pH of lipid acyl hydrolase was 8.2.The lipase activity was activated by Fe^2+,Fe^3+and Mg^2+,inhibited by K^+and Ca^2+.In addition,low concentration of Al^3+and Zn^2+were promoting the hydrolysis ability of lipid acyl hydrolase and the high concentration of Zn^2+inhibits the activity of the lipid acyl hydrolase.The results of this study will provide some new scientific theory guidance for the development and utilization as well as its functional property of the Patatin protein.

关 键 词：马铃薯 酯酰基水解酶 温度 PH 金属离子 

分 类 号：S53[农业科学—作物学]