Measurement of Free and Glucuronidated Cardamonin in Rat Plasma and Bile Using UPLC-MS/MS and Its Application to a Pharmacokinetic and Bile Excretion Study  

作　　者：WU Yi JING Runan JIA Guihua WANG Yixiao YU Tianmiao ZHANG Weidong 

机构地区：[1]School of Pharmaceutical Sciences,Jilin University,Changchun 130021,P.R.China

出　　处：《Chemical Research in Chinese Universities》2019年第6期962-966,共5页高等学校化学研究（英文版）

摘　　要：A rapid and accurate ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-MS/MS)method was established and validated for the measurement of two forms of cardamonin,i.e.,free and glucuronidated,in the plasma and bile of rats.Cardamonin and an internal standard(1,8-dihydroxyanthraquinone)were extracted from plasma and bile with ethyl ether via liquid-liquid extraction.The analytes in the extracts were separated by an Agilent Zorbax Stable Bond-C18 column(2.1 mm×50 mm,1.8μm)under isocratic elution conditions[acetonitrile(A)and 0.1%ammonium formate in water(B),40:60(volume ratio)]with a flow rate of 0.4 mL/min,and mass spectrometry in negative ion MRM mode was implemented for analysis.Good linearity over the wide ranges of 0.01–5μg/mL for plasma and 0.025–10μg/mL for bile samples was acquired.Method validation was performed according to the FDA guidelines for bioanalytical methods.

关 键 词：Glucuronidated CARDAMONIN Pharmacokinetics BILE EXCRETION Ultra-high-performance liquid cliromatography-electrospray ionization-tandem mass spectrometr\(UPLC-MS/MS) 

分 类 号：R28[医药卫生—中药学]