茯砖茶对NAFLD大鼠肠道黏膜屏障及肝损伤的保护作用研究  

Protective Effect of Fu Brick Tea on Intestinal Mucosal Barrier and Liver Injury in NAFLD Rats

作　　者：左高隆 陈美艳 林勇[1,2,3] 林海燕 刘仲华[1,2,3] 吴文亮 ZUO Gaolong;CHEN Meiyan;LIN Yong;LIN Haiyan;LIU Zhonghua;WU Wenliang(Key Lab of Tea Science of Ministry of Education,Hunan Agricultural University,Changsha 410128,China;National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,Changsha 410128,China;Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients,Changsha 410128,China;Tea Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]湖南农业大学茶学教育部重点实验室,湖南长沙410128 [2]国家植物功能成分利用工程技术研究中心,湖南长沙410128 [3]湖南省植物功能成分利用协同创新中心,湖南长沙410128 [4]湖南省农业科学院茶叶研究所,湖南长沙410125

出　　处：《茶叶科学》2019年第6期631-640,共10页Journal of Tea Science

基　　金：湖南省研究生科研创新项目（CX20190518）;作物种质创新与资源利用重点实验室开放研究项目（15KFXM14）

摘　　要：为探讨茯砖茶对高脂诱导的非酒精性脂肪肝病(Non-alcoholic fatty liver disease,NAFLD)大鼠肠黏膜屏障及肝损伤的保护作用,将SD雄性大鼠随机分为正常组、NAFLD组、茯砖茶低、高剂量组,每组8只。通过分析大鼠的采食量、体重变化、血脂指标、肝脏指标及病理切片来评估NAFLD大鼠模型的构建情况及茯砖茶的干预作用。通过分析空肠与结肠病理及PAS染色切片、绒毛高度/隐窝深度值(V/C值)、血清二胺氧化酶(DAO)和脂多糖(LPS)活力评估肠道完整性和通透性;通过分析空肠上皮间淋巴细胞(JIL)数量和血清肿瘤坏死因子(TNF-α)活力评估肠道相关炎症。结果表明,茯砖茶能减少NAFLD大鼠的采食量和体重,降低血脂水平,减少血清中谷草转氨酶(AST)和谷丙转氨酶(ALT)活力,并抑制肝脏的脂质聚集和炎细胞浸润。茯砖茶还降低了血清炎症因子TNF-α和血清LPS的水平,并使血清DAO活力上升。同时,茯砖茶抑制了NAFLD大鼠空肠和结肠绒毛中发生的炎细胞浸润现象,减少了空肠绒毛脱落、断裂、稀疏和紊乱现象,增加了空肠绒毛V/C值,降低了空肠上皮间淋巴细胞数量,增加了空肠和结肠的杯状细胞数量。这些结果说明,茯砖茶能够有效的改善高脂饮食诱导的肠道黏膜屏障及肝损伤,起到防治NAFLD的作用。To explore the protective effect of Fu brick tea on intestinal mucosal barrier and liver injury in rats with high-fat induced non-alcoholic fatty liver disease(NAFLD).SD male rats were randomly divided into normal,NAFLD,low-dose and high-dose Fu brick tea groups,8 rats each group.The establishment of NAFLD rat model and the intervention effect of Fu brick tea were evaluated by analyzing the feed intake,weight change,blood lipid index,liver index and pathological section of rats.Intestinal integrity and intestinal permeability was assessed by pathological and PAS staining sections of jejunum and colon,villus height/crypt depth(V/C value)serum diamine oxidase(DAO)activity and lipopolysaccharide(LPS)activity.Intestinal-related inflammation was assessed by the number of jejunum intraepithelial lymphocytes(JIL)and the activity of serum tumor necrosis factor-alpha(TNF-α).The results show that Fu brick tea could reduce the feed intake andbody weight of NAFLD rats,decrease blood lipid levels,reduce the activity of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum,and inhibit lipid accumulation and inflammation in the liver.Fu brick tea also reduced the levels of serum inflammatory factors TNF-αand serum LPS and increased the activity of serum DAO.Moreover,Fu brick tea inhibited inflammatory cell infiltration in jejunum and colon villi of NAFLD rats,reduced the jejunum villus shedding,fracture,sparse and disorder,increased the jejunum villus V/C value,and decreased the number of jejunum intraepithelial lymphocytes,and increased the number of goblet cells in the jejunum and colon.These data indicate that Fu brick tea could effectively improve the intestinal mucosal barrier and liver injury induced by high-fat diet,which could be used to prevent and treat NAFLD.

关 键 词：茯砖茶 非酒精性脂肪肝病(NAFLD) 肠道屏障 

分 类 号：TS272.54[农业科学—茶叶生产加工] R574[轻工技术与工程—农产品加工及贮藏工程]