Super-resolution microscopy: successful applications in centrosome study and beyond  

作　　者：Jingyan Fu Chuanmao Zhang 

机构地区：[1]State Key Laboratory of Agrobiotechnology,College of Biological Sciences,China Agricultural University,Beijing 100193,China [2]Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education,College of Life Sciences,Peking University,Beijing 100871,China

出　　处：《Biophysics Reports》2019年第5期235-243,共9页生物物理学报（英文版）

基　　金：We thank Dr.Jia Luo for preparing the images shown in Fig.1,Ms.Yuan Tian for the 3D-SIM and STED images shown in Fig.3B,and De Tony Yang and Dr.Jung-Chi Liao for the STORM image shown in Fig.3B.Studies in the Fu Laboratory were supported by the National Natural Science Foundation of China(31722032 and 31771494);the Ministry of Science and Technology of China(2017YFA0506600);whereas the studies in the Zhang Laboratory were supported by the National Natural Science Foundation of China(31430051 and 31520103906);the Ministry of Science and Technology of China(2016YFA0500201 and 2016YFA0100501)。

摘　　要：Centrosome is the main microtubule-organizing center in most animal cells.Its core structure,centriole,also assembles cilia and flagella that have important sensing and motility functions.Centrosome has long been recognized as a highly conserved organelle in eukaryotic species.Through electron microscopy;让s ultrastructure was revealed to contain a beautiful nine-symmetrical core 60 years ago,yet its molecular basis has only been unraveled in the past two decades.The emergence of super-resolution microscopy allows us to explore the insides of a centrosome,which is smaller than the diffraction limit of light.Super-resolution microscopy also enables the compartmentation of centrosome proteins into different zones and the identification of their molecular interactions and functions.This paper compiles the centrosome architecture knowledge that has been revealed in recent years and highlights the power of several super-resolution techniques.

关 键 词：CENTROSOME Super-resolution microscopy SIM STED SMLM 

分 类 号：Q50[生物学—生物化学]