Jaridon 6, a natural autophagy inducer, anti-gastric resistance in vivo and in vitro  

作　　者：FU Ling HAN Bing-kai WANG Tian-ye WANG Jun-wei LIU Xiao-li WANG Jia-min XIONG Ya-lan LI Hui-ju JIA Lu KE Yu LIU Hong-min 

机构地区：[1]School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001,China [2]Key Laboratory of Henan Province for Drug Quality and Evaluation,Zhengzhou 450001,China [3]Key Laboratory of Technology of Drug Preparation,Ministry of Education,Zhengzhou 450001,China [4]Collaborative Innovation Center of New Drug Research and Safety Evaluation,Zhengzhou 450001,China

出　　处：《中国药理学与毒理学杂志》2019年第9期684-685,共2页Chinese Journal of Pharmacology and Toxicology

摘　　要：OBJECTIVE Tumor resistant is the main cause leading to treatment failure.Tumor resistant is related to many aspect of tumor.Jaridon 6 is a new sesquiterpene come from Rabdosia rubescens extacted by our research team previously,have been tested having more obvious advantage than other anti-cancer drugs on tumor-resistant cells including a PTX-resistant gastric cancer cell.But how does the product work?In this paper,we have researched the effect and specific mechanism of Jaridon 6 on gastric-resistant.METHODS In the test,we have adopted vivo and vitro tests to identify the effect of Jaridon 6 on different gastric cancer-resistant cell.The MTT results,Flow test,Western blotting and nude test all verified the anti-cancer of Jaridon 6 on MGC803/PTX gastric resistant cell.We have also used scarifica⁃tion test and transwell tests and Western blotting to testify the inhibition action on EMT by Jaridon 6.The TEM and immu⁃nofluorescence tests have proved the induction autophagy of Jaridon 6.Immunohistochemical tests have been taken to assure the action of Jaridon 6 on EMT,autophagy and PI3K pathway in vivo.RESULTS Jaridon 6 could inhibite the gastric cancer resistant cell in vivo and in vitro.From the mechanism,the inhibiter of 3-MA or LY2940004 have proved the action on PI3K pathway and autophagy by Jaridon 6.Jaridon 6 could induce autophagy in tumor-resistant cells by inhibiting PI3K/AKT pathway.Except that,Jaridon 6 could inhibite the EMT of gastric cancer resistant cell.CONCLUSION Jaridon 6 could be considered for curing gastric cancer resistance,especially for PTX-resistance.Jaridon 6 is a natural autophagy inducer.OBJECTIVE Tumor resistant is the main cause leading to treatment failure. Tumor resistant is related to many aspect of tumor. Jaridon 6 is a new sesquiterpene come from Rabdosia rubescens extacted by our research team previously, have been tested having more obvious advantage than other anti-cancer drugs on tumor-resistant cells including a PTX-resistant gastric cancer cell. But how does the product work? In this paper, we have researched the effect and specific mechanism of Jaridon 6 on gastric-resistant. METHODS In the test, we have adopted vivo and vitro tests to identify the effect of Jaridon 6 on different gastric cancer-resistant cell. The MTT results, Flow test, Western blotting and nude test all verified the anti-cancer of Jaridon 6 on MGC803/PTX gastric resistant cell. We have also used scarifica-tion test and transwell tests and Western blotting to testify the inhibition action on EMT by Jaridon 6. The TEM and immunofluorescence tests have proved the induction autophagy of Jaridon 6. Immunohistochemical tests have been taken to assure the action of Jaridon 6 on EMT, autophagy and PI3 K pathway in vivo. RESULTS Jaridon 6 could inhibite the gastric cancer resistant cell in vivo and in vitro. From the mechanism, the inhibiter of 3-MA or LY2940004 have proved the action on PI3 K pathway and autophagy by Jaridon 6. Jaridon 6 could induce autophagy in tumor-resistant cells by inhibiting PI3 K/AKT pathway. Except that, Jaridon 6 could inhibite the EMT of gastric cancer resistant cell. CONCLUSION Jaridon 6 could be considered for curing gastric cancer resistance, especially for PTX-resistance. Jaridon 6 is a natural autophagy inducer.

关 键 词：tumor resistant MGC803/PTX AUTOPHAGY PI3K/AKT EMT 

分 类 号：R73[医药卫生—肿瘤]