Protective effects of costunolide on mouse brain slice injury induced by oxygen glucose deprivation/reoxygenation  

作　　者：MA Hui-xia HOU Fan CHEN Ai-ling ZHANG Zhuan-zhuan ZHU Ya-fei ZHAO Qi-peng 

机构地区：[1]Key Laboratory of Hui Ethnic Medicine Modernization,Ministry of Education,Ningxia Medical University,Yinchuan 750004,China [2]Department of Pharmacology,Ningxia Medical University,Yinchuan 750004,China

出　　处：《中国药理学与毒理学杂志》2019年第9期693-693,共1页Chinese Journal of Pharmacology and Toxicology

基　　金：National Natural Science Foundation of China(81660700;81260679);Ningxia College FirstClass Discipline Construction Project(Chinese Medicine)Funded Project(NXYLXK2017A06)

摘　　要：OBJECTIVE To investigate the protective effects and mechanisms of costunolide against mousebrain slice injury induced by oxygen-glucose deprivation/reoxygenation(OGD/R).METHODS Mouse brain slice injury was induced by OGD/R in vitro,and the degree ofinjury was evaluated by measuring the release of lactate dehydrogenase(LDH)and 2,3,5-triphenyltetrazolium chloride(TTC)staining.Western blotting was used to analyze the expression of Bax,Bcl-2,Cyt-c,caspase-9,caspase-7 and caspase-3.RESULTS Compared with OGD/R,1,5,and 10μmol·L^-1 costu⁃nolide decreased the LDH levels,increased the TTC staining intensity,inhibited Bax,Cyt-c,caspase-9,caspase-7,caspase-3 expression levels,and enhanced Bcl-2 expression level.CONCLUSION Costunolide has latent neuroprotective activi⁃ties by the regulation of apoptosis via the mitochondrial apoptosis pathway.OBJECTIVE To investigate the protective effects and mechanisms of costunolide against mousebrain slice injury induced by oxygen-glucose deprivation/reoxygenation（OGD/R）. METHODS Mouse brain slice injury was induced by OGD/R in vitro, and the degree ofinjury was evaluated by measuring the release of lactate dehydrogenase（LDH） and 2,3,5-triphenyltetrazolium chloride（TTC） staining. Western blotting was used to analyze the expression of Bax, Bcl-2, Cyt-c, caspase-9, caspase-7 and caspase-3. RESULTS Compared with OGD/R, 1, 5, and 10 μmol·L-1 costunolide decreased the LDH levels, increased the TTC staining intensity, inhibited Bax, Cyt-c, caspase-9, caspase-7, caspase-3 expression levels, and enhanced Bcl-2 expression level. CONCLUSION Costunolide has latent neuroprotective activities by the regulation of apoptosis via the mitochondrial apoptosis pathway.

关 键 词：COSTUNOLIDE ischemic stroke brain slice APOPTOSIS 

分 类 号：R73[医药卫生—肿瘤]