乳酸菌E6-1对人口腔癌细胞Cal-27体外增殖的抑制作用及机制探讨  

Inhibiting effect and mechanism of Lactobacillus E6-1 on oral cancer cell line Cal-27

作　　者：高磊[1] 孙智辉 于浩[1] 井建鑫 徐仲秋 李潇[1] 张楠[1] GAO Lei;SUN Zhi-hui;YU Hao;JING Jian-xin;XU Zhong-qiu;LI Xiao;ZHANG Nan(Department of Oral and Maxillofacial Surgery,The First Hospital of Qiqihar City,Qiqihar 161000,Heilongjiang Province;Department of Oral and Maxillofacial Surgery,The Fifth Affiliated Hospital of Zunyi Medical University,Zhuhai 519100,Guangdong Province,China)

机构地区：[1]齐齐哈尔市第一医院口腔颌面外科,黑龙江齐齐哈尔161000 [2]遵义医科大学第五附属(珠海)医院口腔颌面外科,广东珠海519100

出　　处：《上海口腔医学》2019年第6期605-609,共5页Shanghai Journal of Stomatology

摘　　要：目的:探讨乳杆菌E6-1在体外对人口腔癌细胞Cal-27增殖抑制及凋亡的影响。方法:选择不同浓度(0、10、20、40 mg/mL)的乳杆菌E6-1,作用于人口腔癌细胞Cal-27后,采用MTT实验检测E6-1对Cal-27肿瘤细胞增殖的抑制作用;利用DNA琼脂糖凝胶电泳法检测对凋亡细胞核DNA的影响;利用免疫印迹法检测各组细胞色素c、caspase-9及caspase-3蛋白表达水平。采用SPSS 21.0软件包对数据进行统计学分析。结果:MTT实验结果表明,E6-1对Cal-27有明显的抑制作用,且呈剂量依赖性;不同浓度的E6-1(10、20、40 mg/mL)及原液可诱导细胞核DNA形成"梯状"条带(梯带);免疫印迹结果显示,与空白对照组相比,细胞色素c、caspase-9及caspase-3蛋白水平显著升高(P<0.05)。结论:在体外实验条件下,一定浓度的E6-1可抑制Cal-27细胞增殖,有效诱导细胞凋亡。PURPOSE:To study the effect of Lactobacillus E6-1 on proliferation and apoptosis of human oral cancer cell line Cal-27 in vitro.METHODS:MTT assay was carried out by different concentrations of Lactobacillus E6-1 on Cal-27 to detect the inhibitory effect on cell proliferation,and the effect on DNA was detected by agarose gel electrophoresis.The expression levels of cyt-c,caspase-9 and caspase-3 in each group was detected by Western blot.The data were analyzed with SPSS 21.0 software package.RESULTS:The results of MTT assay showed that E6-1 had obvious inhibitory effect on Cal-27 in a dose-dependent manner.DNA ladder in Cal-27 cells was induced by different concentrations of E6-1(10,20,40 mg/mL).Compared with blank control group,Western blot results showed that the expression of cyt-c,caspase-9 and caspase-3 increased significantly(P<0.05).CONCLUSIONS:Lactobacillus E6-1 can inhibit the proliferation of Cal-27 and effectively induce apoptosis of tumor cells In vitro.

关 键 词：口腔癌 乳杆菌 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]