复合酶法提取鲜玉米皮阿拉伯木聚糖的研究  

Study on Double-enzymatic Extraction of Arabinoxylan from Fresh Corn Fiber

作　　者：马福敏[1] 顾佳 MA Fu-min;GU Jia(Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities,Education Department of Jilin Provincial Government,Changchun University,Changchun 130022,Jilin,China;School of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,Jilin,China)

机构地区：[1]长春大学农产品深加工吉林省教育厅高校重点实验室,吉林长春130022 [2]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品研究与开发》2019年第24期106-109,共4页Food Research and Development

基　　金：国家自然基金青年基金项目（31701611）;吉林省教育厅项目（吉教科验字[2018]328号）

摘　　要：玉米皮作为清洁易得的农产品下脚料,是开发食品组分的丰富资源,其中富含功能性非淀粉多糖--阿拉伯木聚糖(arabinoxylans,AXs)。以鲜玉米麸皮为主要原料,采用复合酶法提取其中的阿拉伯木聚糖。以提取率和提取物中AXs含量为指标,研究复合酶中的木聚糖酶和纤维素酶的比例和复合酶的添加量对得率和提取物中AXs含量的影响。结果表明木聚糖酶与纤维素酶添加量之比为10∶1(酶活比),复合酶的添加量为900 U/g时提取率和提取物中AXs含量最高。Corn fiber is one of the most abundant and clean byproduct of agricultural products,which arouse interest for finding more valuable products for the manufacture of food ingredients from it.The main non-starch polysaccharide of maize bran is arabinoxylans(AXs).In this study fresh corn fiber was used as material,xylanase was selected to composite with cellulase to increase the extraction yield of AXs.The effects of xylanase to cellulase ratio and the double-enzyme dose on the extraction yield and the AXs content in the extract were studied.The results showed that when xylanase to cellulase ratio was 10∶1(U/U),the double-enzyme dose was 900 U/g,the extraction yield and the AXs content in the extract were the highest.

关 键 词：木聚糖酶 纤维素酶 阿拉伯木聚糖(AXs) 提取率 含量 

分 类 号：TS2[轻工技术与工程—食品科学与工程]