鲍鱼内脏蛋白肽的理化性质及其抗氧化活性  

Physicochemical Properties and Antioxidant Activity of Abalone Visceral Peptide

作　　者：李婷 翁武银 Li Ting;Weng Wuyin(College of Food and Biological Engineering,Jimei University,Xiamen 361021,Fujian;Xiamen Key Laboratory of Marine Functional Food,Xiamen 361021,Fujian)

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021 [2]厦门市海洋功能食品重点实验室,福建厦门361021

出　　处：《中国食品学报》2019年第12期76-82,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31571835);海洋公益性行业科研专项(201405016)

摘　　要：对鲍鱼内脏蛋白肽(AVP)的基本成分、分子质量分布和氨基酸组成等理化性质和体外抗氧化活性进行探究。以正常组、模型组和GSH组作为对照,考察低、中、高剂量组(125,250和500 mg/kg AVP)对D-半乳糖致氧化损伤小鼠的影响,评价AVP的体内抗氧化活性。结果显示:AVP除含主成分蛋白外,还含有5.11%多糖和少量的矿物质元素,分子质量主要分布在180~500 u范围。AVP中疏水性氨基酸和支链氨基酸分别占结构氨基酸的49.77%和22.92%,清除羟自由基和DPPH自由基活性的EC50值分别为1.54 mg/mL和1.51 mg/mL。AVP抑制脂质过氧化的效果优于维生素C。AVP能提高D-半乳糖致衰老鼠中血清和肝脏的SOD活性,降低血清的MDA含量,减缓肝脏组织氧化损伤。以上结果表明利用鲍鱼内脏制备的AVP具有良好的抗氧化活性,可作为天然抗氧化剂应用在生物制品和食品工业。The physicochemical properties including proximate compositions,molecular weight distribution and amino acid compositions,and in vitro antioxidant activities of abalone visceral peptide(AVP)were investigated.In vivo antioxidant activities of AVP were evaluated using a D-galactose induced oxidative damage mice model.Animals were divided into normal and model control groups,and low-,middle-,and high-dose groups(125,250,and 500 mg/kg AVP),as well as GSH groups.In addition to the main component of protein,the AVP also contained 5.11%polysaccharide and a small amount of minerals.It was found that the peptide components at molecular weight of 180-500 u were main fractions.The hydrophobic amino acid and branched chain amino acid contents in the complete amino acids of AVP were 49.77%and 22.92%,respectively.The EC50 values of both hydroxyl and DPPH radical scavenging activity of AVP were 1.54 mg/mL and 1.51 mg/mL,respectively.Furthermore,the lipid peroxidation inhibitory effect of AVP was better than that of vitamin C.On the other hand,AVP could improve the activity of superoxide dismutase(SOD)in serum and liver of the aging mice induced by D-galactose,decrease the malondialdehyde(MDA)content in serum,and attenuate the morphological damage in liver tissue.It was concluded that AVP prepared from abalone viscera had good antioxidant activity,and the AVP could be used as a natural antioxidant in the biological product and food industries.

关 键 词：鲍鱼内脏蛋白肽 理化性质 体外抗氧化活性 体内抗氧化活性 

分 类 号：R28[医药卫生—中药学]