免疫组化标记物联合检测在胃肠道间质瘤病理学诊断中的意义  

The significance of combined detection of immunohistochemical markers in the pathological diagnosis of gastrointestinal stromal tumor

作　　者：赵超[1] 张楠[1] 陈凯丽 王硕 张擎 成洪聚 相龙全 Zhao Chao;Zhang Nan;Chen Kaili;Wang Shuo;Zhang Qing;Cheng Hongju;Xiang Longquan(School of Second Clinical Medicine,Jining Medical University,Jining 272067,China;School of Clinical Medicine,Jining Medical University,Jining 272067,China;College of Mental Health,Jining Medical University,Jining 272067,China;Department of Physiology,College of Basic Medicine,Jining Medical University,Jining 272067,China;Department of Pathology,Jining First People's Hospital,Jining 272011,China)

机构地区：[1]济宁医学院第二临床医学院,272067 [2]济宁医学院临床医学院,272067 [3]济宁医学院精神卫生学院,272067 [4]济宁医学院基础医学院生理学教研室,272067 [5]济宁市第一人民医院病理科,272011

出　　处：《国际医药卫生导报》2019年第24期3982-3985,共4页International Medicine and Health Guidance News

基　　金：山东省高等学校科技计划项目资助课题(J18KB119);国家级大学生创新创业训练计划项目(201810443032)。

摘　　要：目的通过分析CD34、CD117、DOG-1、SMA、Demsin及S-100在胃肠道间质瘤中的表达与胃肠道间质瘤临床病理特征的关系,探讨免疫标记物联合检测在其病理诊断中的意义。方法随机抽取2016年1月至2018年12月济宁市第一人民医院65例术后证实为胃肠道间质瘤的病理样本,65例患者均行手术治疗。采用免疫组化方法检测瘤体内CD34、CD117、DOG-1、SMA、Demsin及S-100的表达,结合临床病理资料,分析相关免疫组化标记物的表达情况与肿瘤大小、位置等临床病理因素的关系,并对患者进行随访。结果瘤体内CD34、CD117、DOG-1、SMA、Demsin及S-100的阳性率分别为89.2%、98.5%、96.9%、35.4%、20.0%、16.9%。CD34的表达与肿瘤大小、位置和危险度分级之间有相关(P<0.05),CD117、DOG-1、SMA、Demsin和S-100的表达与各项指标之间无相关,Demsin的表达与性别差异有相关(P=0.018)。结论CD34、CD117及DOG-1在瘤体内的表达具有较高的阳性率,CD117和DOG-1是两种可以辅助CD34诊断胃肠道间质瘤的指标,免疫组化标记物的联合检测有助于明确胃肠道间质瘤诊断。Objective To explore the significance of combined detection of immunohistochemical markers in its pathological diagnosis by analyze the expression of CD34,CD117,DOG-1,SMA,Demsin,and S-100 and their relationship about clinicopathological features in strointestinal stromal tumors.Methods 65 pathological samples of gastrointestinal stromal tumor confirmed after operation from January 2016 to December 2018 were randomly selected from the Jining First People’s Hospital.All of the 65 patients received surgical treatment.The expressions of CD34,CD117,DOG-1,SMA,Demsin,and S-100 in the tumor were detected by immunohistochemical method.The correlation between the expression of relevant immunohistochemical markers and clinicopathological factors such as tumor size and location were analyzed based on the clinicopathological data,and the patients were followed-up.Results The positive rates of CD34,CD117,DOG-1,SMA,Demsin,and S-100 were 89.2%,98.5%,96.9%,35.4%,20.0%,and 16.9%,respectively.The expression of CD34 was related to tumor size,location,and risk level(P<0.05),while the expression of CD117,DOG-1,SMA,Demsin,and S-100 were not related,and the expression of Demsin was related to gender(P=0.018).Conclusions The expression of CD34,CD117,and DOG-1 in the tumor have relatively high positive rates.CD117 and DOG-1 are two indexes that could assist CD34 in the diagnosis of gastrointestinal stromal tumor,and the combined detection of immunohistochemical markers is helpful in the diagnosis of gastrointestinal stromal tumor.

关 键 词：胃肠道间质瘤 免疫组化 病理学 联合检测 

分 类 号：R73[医药卫生—肿瘤]