TAT-Apoptin基因原核表达载体的构建及其表达鉴定  

Construction and expression Identification of prokaryotic expression Vector of TAT-Apoptin Gene

作　　者：陈桂华 李翔辉[1,2] 郁川 Chen Guihua;Li Xianghui;Yu Chuan(Luohe Food Vocational College,462300;Henan University Of Science and Technology,471003)

机构地区：[1]漯河食品职业学院,462300 [2]河南科技大学动物疫病与公共安全重点实验室,471003

出　　处：《现代农业研究》2019年第11期96-98,共3页Modern Agriculture Research

摘　　要：为得到TAT-Apoptin融合蛋白,以pMD-19T-Apoptin为模板PCR扩增出TATApoptin基因,构建重组载体pET-32a-TAT-Apoptin,并对TAT-Apoptin蛋白进行表达。PCR和测序表明,成功构建重组载体pET-32a-TAT-Apoptin,进一步对其进行蛋白表达,发现该蛋白能与Apoptin多抗发生特异性结合。以上结果证明,TAT-Apoptin基因原核表达载体构建成功,并能在Rosetta中进行分泌性表达。In order to obtain TAT-Apoptin fusion protein,TAT-Apoptin gene was amplified by PCR with pMD-19T-Apoptin as template,the recombinant vector pET-32a-TAT-Apoptin,was constructed and the TAT-Apoptin protein was expressed.PCR and sequencing showed that the recombinant vector pET-32a-TAT-Apoptin,was successfully constructed to further express the protein,and it was found that the protein could specifically bind to Apoptin polyantibodies.The above results show that TATapoptin base The prokaryotic expression vector was successfully constructed and secreted in Rosetta.

关 键 词：TAT-Apoptin融合蛋白 重组载体pET-32a-TAT-Apoptin 分泌性表达 

分 类 号：R73[医药卫生—肿瘤]