山东北虫草不同极性部位体外抗氧化活性研究  

The Antioxidant Activity in Vitro in Different Polar Parts of Cordyceps Militaris in Shandong

作　　者：李万芳 余登升 刘敬民 LI Wanfang;YU Dengsheng;LIU Jingmin(School of Preschool Education,Shandong Yingcai University,Jinan,Shandong,China 250104;Shandong Academy of Pesticide Sciences,Jinan,Shandong,China 250033)

机构地区：[1]山东英才学院学前教育学院,山东济南250104 [2]山东省农药科学研究院,山东济南250033

出　　处：《中国药业》2020年第1期23-25,共3页China Pharmaceuticals

摘　　要：目的探讨山东北虫草不同极性部位体外抗氧化活性。方法采用邻苯三酚自氧化法和1,1-二苯基-2-三硝基苯肼(DPPH自由基)清除能力测定法检测北虫草不同极性部位的体外抗氧化活性。结果北虫草水提取部位、乙酸乙酯部位清除超氧阴离子和DPPH自由基的作用与维生素C相比,差异无统计学意义(P>0.05);北虫草不同极性部位清除超氧阴离子和DPPH自由基的能力与浓度呈正相关;北虫草不同极性部位清除超氧阴离子能力的大小为水提取部位>乙酸乙酯部位>石油醚部位>正丁醇部位,清除DPPH自由基能力的大小为水提取部位>乙酸乙酯部位>正丁醇部位>石油醚部位。结论北虫草水提取部位和乙酸乙酯部位有较强的体外抗氧化活性作用。Objective To examine the antioxidant activities in vitro in different polar parts of Shandong Cordyceps militaris.Methods Pyrogallol self-oxidation and DPPH free radical scavenging were used to examine the antioxidant activities in vitro.Results The effects of scavenging superoxide anion and DPPH free radical in the water extracting fraction and ethyl acetate fraction of Cordyceps militaris were similar to those of Vc(P>0.05).The different polar parts of Cordyceps militaris had certain ability to scavenge superoxide anion and DPPH free radicals at different concentrations,and the clearance rate was positively correlated with the concentration.The ability of removing the superoxide anion from different polar parts of Cordyceps militaris was water extraction site>ethyl acetate site>petroleum ether site>n-butanol site;the ability to remove DPPH free radicals was water extraction site>ethyl acetate site>n-butanol site>petroleum ether site.Conclusion The water extracting part and ethyl acetate part of Cordyceps militaris have strong antioxidant activity in vitro.

关 键 词：北虫草 极性部位 抗氧化活性 邻苯三酚自氧化法 1 1-二苯基-2-三硝基苯肼 

分 类 号：R932[医药卫生—生药学] R285.5[医药卫生—药学]