3种鲎试验在乳品内毒素活性定量检测中的应用评价  

Application evaluation of 3 Tachypleus amebocyte lysate methods for quantitative detection of endotoxin activities in dairy products

作　　者：张灿 白淼 张明露[2] 章雷 史云 王莉莉 ZHANG Can;BAI Miao;ZHANG Ming-Lu;ZHANG Lei;SHI Yun;WANG Li-Li(Center for Disease Control and Prevention of Chinese PLA,Beijing 100071,China;Department of Environmental Science and Engineering,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]中国人民解放军疾病预防控制中心,北京100071 [2]北京工商大学环境科学与工程系,北京100048

出　　处：《食品安全质量检测学报》2019年第23期7996-8003,共8页Journal of Food Safety and Quality

基　　金：国家重点研发计划(2016YFD0401202)~~

摘　　要：目的比较3种内毒素活性定量检测方法在乳品中的应用情况。方法采用2种传统东方鲎(Tachypleus amebocyte lysate,TAL)试验(动态浊度法/动态显色法)和1种便携式内毒素检测仪(portabletest system, PTS)对牛奶、酸奶和奶粉产品内毒素活性进行检测,评估3种方法在乳品内毒素活性检测中的应用情况。结果 3种方法用于乳品内毒素活性检测的加标回收率为50%~200%,符合药典要求。对比动态浊度法,动态显色法检测乳品内毒素活性的加标回收率范围更加接近100%。PTS方法的内毒素活性检测值分别与2种传统TAL试验的检测值有较好的线性关系。结论 3种方法均适用于乳品内毒素活性的检测,但动态显色法更加适合乳品内毒素的检测。Objective To compare the application of 3 methods for quantitative determination of endotoxin activity in dairy products. Methods Endotoxin activities in milk, yogurt and milk powder products was determined using 2 traditional Tachypleus amebocyte lysate(TAL) tests(kinetic-turbid/kinetic-chromogenic assay) and a portable test system(PTS). The test was carried out to evaluate the application of the 3 methods in the detection of endotoxin activity in dairy products. Results The recovery ratios of 3 methods for the detection of endotoxin activity in dairy products are 50%-200%, which met the requirements of pharmacopoeia. Compared with kinetic-turbid assay, the recovery ratios of kinetic-chromogenic assay for the detection of endotoxin activity in dairy products was closer to 100%. The endotoxin activity detection value of the PTS method had a good linear relationship with the detection values of the 2 traditional TAL assay. Conclusion All the 3 methods can be used for endotoxin determination in dairy products, and the kinetic-chromogenic assay is more suitable than others.

关 键 词：乳品 内毒素活性 鲎试验 动态浊度法 动态显色法 便携式内毒素检测仪 

分 类 号：R28[医药卫生—中药学]