实时直接分析质谱法快速筛查南美白对虾中磺胺类药物  

Rapid screening of sulfonamides in penaeusvannamei by using direct analysis in real time coupled to Q-orbitrap mass spectrometry

作　　者：齐春艳 王立亚 曾雪芳 刘嘉飞[1] 叶靖怡 林洁纯 温海滨 雷毅 梁旭霞 冼燕萍 QI Chun-Yan;WANG Li-Ya;ZENG Xue-Fang;LIU Jiang-Fei;YE Jing-Yi;LIN Jie-Chun;WEN Hai-Bin;LEI Yi;Liang Xu-Xia;XIAN Yan-Ping(Guangdong Institute of Food Inspection(Guangdong Inspection Center of Wine and Spirits),Guangzhou 510435,China;Guangzhou Quality Supervision and Testing Institute,Guangzhou 511447,China)

机构地区：[1]广尔省食品检验所(广东省酒类检测中心),广州510435 [2]广州质量监督检测研究院,广州511447

出　　处：《食品安全质量检测学报》2019年第23期8048-8055,共8页Journal of Food Safety and Quality

基　　金：广东省食品药品监督管理局科技创新项目(2018TDB07)~~

摘　　要：目的建立了一种实时直接分析离子源(direct analysis in real time, DART)结合四极杆/静电轨道离子阱高分辨质谱法(Q-Exactive Orbitrap)快速筛查南美白对虾中磺胺类药物的方法。方法样品经过提取净化后,在离子化温度450℃,栅极电压350 V,样品传输速度0.2 mm/s的DART离子源正离子模式条件下进样,Orbitrap高分辨质谱在-全扫描/数据依赖二级扫描模式下进行定性检测。结果该方法检出限为0.5~2.7μg/kg,满足筛查要求。结论该方法分析速度快,定性准确,无需色谱分离过程,环境友好。Objective To establish a method for screening of sulfonamides in penaeusvannamei by a direct analysis in real time coupled to orbitrap mass spectrometry. Methods After extraction and purification, the samples were injected under the conditions of ionization temperature of 450 ℃, the grid electrode voltage of 350 V and the sampling rate of 0.2 mm/s using DART in the positive ion mode. The samples were analyzed using DART-Orbitrap MS in full scan mode. Results The limits of detection were in the range of 0.5-2.7 μg/kg, and it can satisfy the screening requirements. Conclusion The DART-Orbitrap MS method have advantages of fast analysis speed, accurate qualitative analysis, no need of chromatographic separation and environmentally friendly.

关 键 词：实时直接分析 静电轨道离子阱质谱 南美白对虾 磺胺类药物 快速筛查 

分 类 号：O65[理学—分析化学]