超声辅助萃取辣木茎生物碱工艺及抑制α-葡萄糖苷酶作用研究  

Ultrasonic-assisted extraction of alkaloids from Moringa oleifera and its inhibitory activity on α-glucosidase

作　　者：姜翠翠 李颖 邱松山 周英彪 JIANG Cuicui;LI Ying;QIU Songshan;ZHOU Yingbiao(Development Centre of Technology for Fruit&Vegetable Storage and Processing Engineering,School of Biology and Food Engineering,Guangdong University of Petrochemical Technology,Maoming 525000,China)

机构地区：[1]广东石油化工学院生物与食品工程学院果蔬加工与贮藏工程技术开发中心

出　　处：《食品与发酵工业》2019年第22期97-102,共6页Food and Fermentation Industries

基　　金：广东省科技计划项目(2017A020225043;2017A090905016);广东高校果蔬加工与贮藏工程技术开发中心开放基金(2012gczxB001)

摘　　要：为进一步促进辣木药用价值的开发利用,该实验采用超声辅助萃取辣木茎中生物碱类物质,利用响应面试验优化萃取参数,分析辣木茎中的生物碱对α-葡萄糖苷酶的体外抑制能力。结果表明,超声辅助萃取辣木茎中的生物碱类物质的最佳工艺参数为超声功率250 W、超声波时间30 min、料液比1∶9(g∶m L),在此条件下辣木茎生物碱提取率为32.78 mg/g,提取率较优化前提高了约33%。辣木茎生物碱对α-葡萄糖苷酶活性的抑制作用优于阿卡波糖,主要以剂量依赖性、时间依赖性的方式抑制α-葡萄糖苷酶,120 mg/L的生物碱对α-葡萄糖苷酶抑制率达到86.11%,抑制作用的IC50为0.034 g/L,其抑制属于竞争性和可逆性抑制。因此基于辣木茎生物碱对α-葡萄糖苷酶具有较强的抑制作用,可将其进一步用于天然α-葡萄糖苷酶抑制剂的开发。Ultrasonic-assisted extraction of total alkaloid from Moringa oleifera was optimized to improve its medicinal value.The inhibitory activity of the alkaloid onα-glucosidase was determined by in vitro experiment.Under optimum extraction conditions:250 W of ultrasonic power,30 min of extraction time and 1∶9 of solid-liquid ratio,the extraction rate of alkaloids was 32.78 mg/g,which was increased by about 33%.The alkaloid extracted from M.oleifera exhibited inhibiting activities onα-glucosidase in a dose-and time-dependent manner with half-inhibitory concentration(IC50)of 34 mg/L,while 86.11%inhibition was obtained at 120 mg/L.The inhibition was reversible and competitive.The results lay a foundation for the development of M.oleifera alkaloid as a natural source ofα-glucosidase inhibitor.

关 键 词：辣木 生物碱 超声波辅助萃取 Α-葡萄糖苷酶 

分 类 号：R28[医药卫生—中药学]