硒、β-胡萝卜素联合应用对大鼠生长过程及抗氧化能力的影响  

Effects of selenium and β-carotene combined on growth process and antioxidant capacity of rats

作　　者：马诚 刘昱[1] 张格祥[1] 刘清[2] MA Cheng;LIU Yu;ZHANG Ge-xiang;LIU Qing(Department of Nutrition and Food Hygiene,School of Public Health of Lanzhou University,Lanzhou 730000;Lanzhou Municipal Center for Disease Control and Prevention,Lanzhou 730000,China)

机构地区：[1]兰州大学公共卫生学院营养与食品卫生学研究所,甘肃兰州730000 [2]兰州市疾病预防控制中心,甘肃兰州730000

出　　处：《西安交通大学学报（医学版）》2020年第1期141-144,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：2015年度兰州市人才创新创业项目(No.2015-RC-72)~~

摘　　要：目的研究单独/联合给予硒、β-胡萝卜素对大鼠生长过程及抗氧化能力的影响,为氧化损伤类慢性病早期预防与治疗中二者的联合应用提供基础数据参考。方法7周龄大鼠随机分组:Se组[亚硒酸钠0.3 mg/(kg·d)],β-C组[β-胡萝卜素10 mg/(kg·d)],Se+β-C组[亚硒酸钠0.3 mg/(kg·d)+β-胡萝素10 mg/(kg·d)],灌胃干预4周,对照组灌服等量水+玉米油。每48 h称量1次大鼠体质量;4周后,采用全自动生化分析仪检测血清中ALT、AST的变化;ELISA法检测血清及肝组织中的氧化应激水平及抗氧化指标。结果各实验组大鼠体质量逐渐增长、血清ALT、AST水平与对照组比较差异无统计学意义(P>0.05);各组大鼠血清T-SOD、GSH-Px活力、MDA含量与对照组比较差异无统计学意义(P>0.05);β-C明显提高了大鼠肝组织T-SOD、GSH-Px活力(P<0.05)。结论单独/联合给予大鼠硒、β-胡萝卜素未对其正常生长过程造成不良影响,β-胡萝卜素干预提高了大鼠肝组织抗氧化能力,对辅助氧化损伤类慢性病早期预防与治疗有一定积极作用。Objective To study the effects of selenium andβ-carotene alone or in combination on the growth process and antioxidant capacity of rats so as to provide basic data for the joint application of the two in early prevention and treatment of chronic diseases caused by oxidative damage.Methods Rats aged 7 weeks were randomly divided into four groups:Se group[sodium selenite 0.3 mg/(kg·d)],β-C group[β-carotene 10 mg/(kg·d)],Se+β-C group[sodium selenite 0.3 mg/(kg·d)+β-carotene 10 mg/(kg·d)].The rats were intragastrically administered for 4 weeks,and those in the control group were intragastrically administered with the same volume of normal saline and corn oil.The rats were weighed every two days.Four weeks later,the changes of ALT and AST in the serum were detected by the automatic biochemical analyzer;the indexes of oxidation(MDA)and antioxidants(SOD,CAT and GSH)in the serum or liver tissue were determined by enzyme-linked immunosorbent assay(ELISA).Results The activities of T-SOD and GSH-Px,the content of MDA in the serum of rats did not significantly differ between the experimental group and the control group(P>0.05).β-C significantly enhanced the activities of T-SOD and GSH-Px in rat liver tissue(P<0.05).Conclusion Selenium andβ-caroten alone or in combination have no harmful effect on the normal growth process of rats.The latter improves the anti-oxidative ability of rat liver tissue,and it has certain positive effects on the early prevention and treatment of chronic diseases.

关 键 词：硒 Β-胡萝卜素 WISTAR大鼠 抗氧化 

分 类 号：R151.2[医药卫生—营养与食品卫生学]