改良TLC-生物自显影引导裸脚菇中抗青、绿霉物质的分离  

Isolation of Substances of Inhibited Citrus Blue and Green Mold from Gymnopus sp. by Developed TLC-Bioautography

作　　者：吴天福 霍光华[1] 韩启灿[1] 吴胜 邹长江 Wu Tianfu;Huo Guanghua;Han Qican;Wu Sheng;Zou Changjiang(Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources,College of Biological Science and Engineering,Jiangxi Agricultural University,Nanchang 330045)

机构地区：[1]江西农业大学生物科学与工程学院菌物资源保护与利用江西省重点实验室

出　　处：《中国食品学报》2020年第1期268-274,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目（31560574）;江西省自然科学基金项目（20171ACB20014）

摘　　要：目的:分离和追踪野生裸脚菇属(0612-9)中抗青、绿霉活性物质。方法:采用正相硅胶柱色谱、ODS柱色谱、半制备HPLC等分离活性成分,用改良薄层色谱-生物自显影(即浇注法)追踪活性成分,牛津杯法、薄层色谱-紫外荧光法、10%浓硫酸乙醇显色等方法作为验证方法。结果:共制备5种化合物,分别为Ⅰ1.1、Ⅰ1.2、Ⅰ2、Ⅰ3和Ⅱ,改良TLC-生物自显影追踪到4种抗菌活性物质,即Ⅰ1.1、Ⅰ2、Ⅰ3和Ⅱ,样品检测量低至18.6μg/mL。结论:本方法具有清晰、准确的优点,适于引导中度极性活性物质,如该裸脚菇抗菌活性物质等的分离。Objective:To isolate and track the active substances against citrus blue and green mold from wide Gymnopus sp.(0612-9).Methods:The phase silica gel column chromatography,ODS column chromatography and semi-preparative HPLC were adopted to separate its active constituents.A developed TLC-bioautography(agar casting)was invented to track these active constituents with verification from the Oxford cup method,TLC-UV fluorescence method,colorimetric method with 10%sulfuric acid-ethanol.Results:Five substances were prepared.There were four antifungal active substances(Ⅰ1.1,Ⅰ2,Ⅰ3 andⅡ)tracked by the way of the developed TLC-bioautography.Detect able amount was even less than 18.6μg/mL.Conclusion:The method has the advantages of high clarity and accuracy,and is suitable for guiding the separation of moderately polar active substances such as the antifungal active substances from Gymnopus sp.

关 键 词：薄层色谱浇注法生物自显影 抗菌物质 分离 裸脚菇 柑橘青、绿霉菌 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]