蜂王浆主蛋白对围绝经期小鼠生殖功能的保护作用  

Protective effects of major royal jelly proteins on reproductive function of mice during perimenopausal period

作　　者：刘欣 陈勇 沈立荣[1] LIU Xin;CHEN Yong;SHEN Lirong(Zhejiang Key Laboratory for Agro-Food Processing,Fuli Institute of Food Science,School of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学生物系统工程与食品科学学院馥莉食品科学研究院浙江省农产品加工技术研究重点实验室

出　　处：《浙江大学学报（农业与生命科学版）》2019年第6期751-759,共9页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金面上项目（31271848）;浙江省科技计划项目（2017C32033）;杭州市重大创新科技项目（20131812A25)

摘　　要：为研究蜂王浆主蛋白(major royal jelly proteins,MRJPs)对围绝经期小鼠生殖内分泌的影响,选取11~13月龄自然衰老雌性ICR小鼠作为动物模型开展实验。将50只小鼠随机分为蜂王浆主蛋白低、中、高剂量组(分别给予125、250、500 mg/kg MRJPs)、阳性对照组(给予125 mg/kg酪蛋白)和老年模型组(给予生理盐水),另选10只5月龄雌性小鼠作为青年对照组(给予生理盐水),所有小鼠每日灌胃1次,持续7周后,计算小鼠子宫指数和卵巢指数,并通过放射免疫技术检测血清激素雌二醇(estradiol,E2)、孕酮(progesterone,P)、促卵泡生成素(folliclestimulating hormone,FSH)和促黄体生成素(luteinizing hormone,LH)含量;此外,采用定量聚合酶链式反应检测小鼠卵巢中激素受体表达量,并在光学显微镜下对卵巢和子宫组织形态进行观察。结果表明:与老年模型组相比,补充中、高剂量的MRJPs可显著提高小鼠卵巢指数和子宫指数,显著提高血清激素E2和P的水平,降低FSH和LH含量,并提高雌激素受体基因ERα、ERβ和孕激素受体基因PR的表达量,增加子宫内膜平均厚度,改善卵巢的卵泡发育和形态。表明MRJPs具有类雌激素效应,对围绝经期小鼠生殖功能具有一定的保护作用。本研究为蜂王浆和MRJPs应用于预防和改善女性围绝经期综合征提供了科学依据。In order to study the effects of the major royal jelly proteins(MRJPs)on the endocrine endocrinology in female mice during the perimenopausal period,an experiment was conducted for the natural aging female ICR mice with the age of 11-13 months.Fifty female mice were randomly divided into five equal groups,including low,middle and high dose groups of MRJPs(administrated with 125,250,500 mg/kg MRJPs,respectively),positive control group(administrated with 125 mg/kg casein)and old model group(administrated with physiological saline).Meanwhile,ten female mice with the age of five months were set as the young control group(administrated with physiological saline).Intragastric administrations were conducted for seven weeks once daily continuously.The indexes of uterus and ovary were calculated,and serum levels of estradiol(E2),progesterone(P),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)were detected by radioimmunoassay.The real-time polymerase chain reaction was used to detect the expressions of estrogen receptorα(ERα),estrogen receptorβ(ERβ)and progesterone receptor(PR).The histomorphological observation of the uterus and ovary was also carried out under an optical microscope.The results showed that MRJPs supplement in the medium and high dosages could increase the uterus index and ovary index.Serum levels of E2 and P were significantly elevated in the MRJPs groups,while the levels of FSH and LH were decreased as compared with the old model group.The expressions of ovarian ERα,ERβand PR were up-regulated significantly in the MRJPs groups.Follicular development was also improved.In conclusion,the above results suggest that MRJPs possess the protective activity in reproduction of mice during the perimenopausal period.It provides scientific evidences of royal jelly and MRJPs in prevention and improvement of perimenopause syndrome.

关 键 词：蜂王浆主蛋白 小鼠 围绝经期综合征 生殖功能 卵巢 血清激素 类雌激素效应 

分 类 号：R151.3[医药卫生—营养与食品卫生学] S896.3[医药卫生—公共卫生与预防医学]