响应面法优化辣木叶中水溶性蛋白提取工艺研究  

Optimization of Extraction Process for Water-soluble Protein of Moringa oleifera Leaves by Response Surface Methodology

作　　者：代佳和 罗旋飞 史崇颖[1] 赵存朝 盛军[1,2] 田洋[1,3] 陶亮 DAI Jiahe;LUO Xuanfei;SHI Chongying;ZHAO Cunchao;SHENG Jun;TIAN Yang;TAO Liang(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Yunnan Research Center for Advanced Tea Processing,Yunnan Agricultural University,Kunming 650201,China;National Research and Development Center for Moringa Processing Technology,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南农业大学,云南省茶深加工工程技术研究中心,云南昆明650201 [3]国家辣木加工技术研发专业中心,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2020年第1期130-138,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省教育厅科学研究基金项目(2016YJS061);国家自然科学基金项目(31560431);中国木薯产业技术体系(CARS-11-YNSJ);农业部热带技术试验示范项目(K2500053)

摘　　要：【目的】辣木总蛋白含量高,但溶解差,水溶性蛋白提取率低,本研究采用超声波辅助酸溶法提取辣木叶中水溶性蛋白。【方法】以辣木叶为原料,分析超声波功率、超声时间、提取温度、水浴时间和溶液pH值6个因素对其水溶性蛋白提取率的影响,在单因素试验的基础上,通过响应面法优化酸法提取辣木蛋白的最佳工艺条件。【结果】响应面优化结果表明:对蛋白提取率的影响依次为溶液pH>料液比>超声时间>水浴温度,最优提取条件为料液比1∶70(g∶mL)、超声波功率300 W、超声时间30 min、浸提水浴温度53℃、溶液pH 1.4、水浴浸提时间60 min,此条件下的水溶性蛋白提取率最高,可达(79.36±1.13)mg/g,与预测值80.194 mg/g基本相符。【结论】本研究提高了水溶性蛋白的提取率,对辣木产业发展及蛋白系列产品开发及蛋白活性研究具有一定的促进意义。[Purpose]Moringa oleifera protein content is high,but is poor water solubility,and low extraction rate.To study the extraction and increase the extraction rate of soluble protein from Moringa leaves.[Method]Application of acid dissolution and ultrasonic wave assisted extraction of protein was studied by using dry Moringa leaves as a raw material by single factor experiment and response surface methodology to determine the best extraction process.The extraction of protein was investigated with response to six main variables,including ultrasonic power,ultrasonic time,extraction temperature,bath time and pH value.[Result]The best extraction conditions were determined by Box-Behnken center united experimental design as follows:the ratio for material and liquid was 1∶70(g∶mL),ultrasonic power of 300 W,Ultrasound time of 30 min,bath temperature of 53℃,pH value of 1.4,bath time of 60 min,the order of influence on the protein extraction rate was alkali solution pH>liquid material ratio>ultrasonic temperature>ultrasonic time,under which the yield of protein in the extracts reached(79.36±1.13)mg/g,consistent with the response surface prediction of 80.194 mg/g.[Conclusion]The extraction rate of water-soluble protein was improved by this method,which was certain significance for the development of Moringa industry and the development of protein series products.

关 键 词：辣木叶 超声波 水溶性蛋白质 提取率 

分 类 号：S794.9[农业科学—林木遗传育种] TS201.2[农业科学—林学]