右旋糖酐蔗糖酶纯化及生产应用的研究进展  

Research Progress on Purification,Production and Application of Dextransucrase

作　　者：徐欣东 王清[1,2] 齐鹏翔[1,2] 黄双霞 梁欣泉 XU Xin-dong;WANG Qing;QI Peng-xiang;HUANG Shuang-xia;LIANG Xin-quan(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;Engineering Research Center for Sugar and Comprehensive Utilization,Ministry of Education,Nanning 530004,China)

机构地区：[1]广西大学轻工与食品工程学院,南宁530004 [2]糖业及综合利用教育部工程研究中心,南宁530004

出　　处：《中国调味品》2020年第1期196-200,共5页China Condiment

基　　金：广西科技重大专项项目（桂科AA17204034）;国家自然科学基金地区科学基金项目（21768001）

摘　　要：右旋糖酐蔗糖酶是一种主要由肠膜明串珠菌分泌获得,能以蔗糖为底物合成右旋糖酐的葡萄糖基转移酶。在右旋糖酐的合成中,酶合成法相比菌种发酵法具有高效连续生产、产物易分离等优点,因此对右旋糖酐蔗糖酶的深入研究将有助于提升酶法合成右旋糖酐、低聚糖等产物的效率和可行性。文章以右旋糖酐蔗糖酶为中心点,综述了其催化机理、纯化方法、生产改造和应用研究进展,并阐述了基因工程、双酶联用等技术对该酶功能的优化,最后针对目前仍存在的问题及未来的研究方向进行了总结。Dextransucrase is a kind of glucosyltransferase which can synthesize dextran using sucrose as the substrate,it's mainly secreted by Leuconostoc mesenteroides.In the synthesis of dextran,the enzyme synthesis method has the advantages of efficient continuous production and easy separation of products compared with strain fermentation method.Therefore,the in-depth study on dextransucrase will help to improve the efficiency and feasibility of enzymatic synthesis of dextran,oligosaccharide and other products.Focus on the dextransucrase,this paper reviews the research progress of its catalytic mechanism,purification methods,production,modification and application,discusses the optimization of the functions of genetic engineering and double-enzyme combination and other technologies on the enzyme,finally,summaries the existing problems and future research directions.

关 键 词：右旋糖酐蔗糖酶 肠膜明串珠菌 右旋糖酐 纯化 应用 

分 类 号：TS201.25[轻工技术与工程—食品科学]