Synaptic development of layer V pyramidal neurons in the prenatal human prefrontal neocortex: a Neurolucida-aided Golgi study  

作　　者：Li-Xin He Lily Wan Wei Xiang Jian-Ming Li An-Hua Pan Da-Hua Lu 

机构地区：[1]Xiangtan Medicine and Health Vocational College,Xiangtan,Hunan Province,China [2]Department of Neurology,Xiangya Hospital,Central South University,Changsha,Hunan Province,China [3]Changde Vocational Technical College,Changde,Hunan Province,China [4]Department of Anatomy,Changsha Medical University,Changsha,Hunan Province,China [5]Department of Anatomy and Neurobiology,Xiangya School of Medicine,Central South University,Changsha,Hunan Province,China

出　　处：《Neural Regeneration Research》2020年第8期1490-1495,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81873780(to DHL);grants from the Department of Education of Hunan Province of China,No.16C1577(to LXH);the Xiangtan Medicine and Health Vocational College of China

摘　　要：The prefrontal neocortex is involved in many high cognitive functions in humans.Deficits in neuronal and neurocircuitry development in this part of the cerebrum have been associated with various neuropsychiatric disorders in adolescents and adults.There are currently little available data regarding prenatal dendrite and spine formation on projecting neurons in the human prefrontal neocortex.Previous studies have demonstrated that Golgi silver staining can identify neurons in the frontal lobe and visual cortex in human embryos.In the present study,five fetal brains,at 19,20,26,35,and 38 gestational weeks,were obtained via the body donation program at Xiangya School of Medicine,Central South University,China.Golgi-stained pyramidal neurons in layer V of Brodmann area 46 in fetuses were quantitatively analyzed using the Neurolucida morphometry system.Results revealed that somal size,total dendritic length,and branching points of these neurons increased from 26 to 38 gestational weeks.There was also a large increase in dendritic spines from 35 to 38 gestational weeks.These findings indicate that,in the human prefrontal neocortex,dendritic growth in layer V pyramidal neurons occurs rapidly during the third trimester of gestation.The use of human fetal brain tissue was approved by the Animal Ethics Committee of Xiangya School of Medicine,Central South University,China(approval No.2011-045)on April 5,2011.The prefrontal neocortex is involved in many high cognitive functions in humans. Deficits in neuronal and neurocircuitry development in this part of the cerebrum have been associated with various neuropsychiatric disorders in adolescents and adults. There are currently little available data regarding prenatal dendrite and spine formation on projecting neurons in the human prefrontal neocortex. Previous studies have demonstrated that Golgi silver staining can identify neurons in the frontal lobe and visual cortex in human embryos. In the present study, five fetal brains, at 19, 20, 26, 35, and 38 gestational weeks, were obtained via the body donation program at Xiangya School of Medicine, Central South University, China. Golgi-stained pyramidal neurons in layer V of Brodmann area 46 in fetuses were quantitatively analyzed using the Neurolucida morphometry system. Results revealed that somal size, total dendritic length, and branching points of these neurons increased from 26 to 38 gestational weeks. There was also a large increase in dendritic spines from 35 to 38 gestational weeks. These findings indicate that, in the human prefrontal neocortex, dendritic growth in layer V pyramidal neurons occurs rapidly during the third trimester of gestation. The use of human fetal brain tissue was approved by the Animal Ethics Committee of Xiangya School of Medicine, Central South University, China(approval No. 2011-045) on April 5, 2011.

关 键 词：Golgi staining human brain banking NEURODEVELOPMENT Neurolucida neuropsychiatric disorders prefrontal cortex SYNAPTOGENESIS three-dimensional reconstruction 

分 类 号：R446[医药卫生—诊断学] R741[医药卫生—临床医学]