Al3+荧光增敏快速检测酸奶和乳酸菌饮料中的胭脂虫红酸（英文）  

Rapid Detection of Carminic Acid in Yogurt and Lactobacillus-Containing Beverage by Al3+-Based Fluorescence Enhancement

作　　者：刘兰香[1] 伊冠东 李凯[1] 马金菊[1] 徐涓[1] 孙彦琳[2] 张弘[1] LIU Lanxiang;YI Guandong;LI Kai;MA Jinju;XU Juan;SUN Yanlin;ZHANG Hong(Research Institute of Resources Insects,Chinese Academy of Forestry,Kunming 650224,China;Faculty of Chemical Engineering,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650224 [2]昆明理工大学化学工程学院,云南昆明650500

出　　处：《食品科学》2020年第2期306-314,共9页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2016YFD0600806);中央级公益性科研院所基本科研业务费专项(CAFYBB2017MA012);云南大学化学工程学院重点实验室开放课题(YNFC19-08)

摘　　要：研究溶剂、浓度、pH值以及金属离子对胭脂虫红酸荧光性能的影响,表明胭脂虫红酸在pH 4且其物质的量浓度为2.0×10^-4 mol/L的水溶液中荧光最强;而胭脂虫红酸对金属离子选择性识别的结果显示,Al^3+能使胭脂虫红酸的荧光强度显著增强从而提高检测的灵敏度。以Al^3+(2.0×10^-3 mol/L)为标准溶液检测样品中胭脂虫红酸含量时,胭脂虫红酸的物质的量浓度在1.0×10^-5~8.0×10^-5 mol/L范围内与其荧光强度呈良好的线性关系,检测限为5.0×10^-6 mol/L。实际应用结果表明,用荧光法检测不同酸奶和乳酸菌饮料中添加的胭脂虫红酸的含量,样品回收率在96.6%~110.5%之间,相对标准偏差小于5.9%。本研究为快速、有效、定量地检测食品中的胭脂虫红酸提供了一种有效的方法。The effects of solvents,carminic acid concentration,pH and metal ions on the fluorescence properties of carminic acid were systematically studied.The fluorescent intensity of carminic acid was the strongest at a concentration of 2.0×10^-4 mol/L in an aqueous solution at pH 4.The selectivity of carminic acid to metal ions indicated that the addition of Al3+could significantly enhance the fluorescence intensity of carminic acid thus improving the sensitivity of detection.A 2.0×10^-3 mol/L aqueous solution of Al3+(at)was used as the standard solution.Thefluorescence intensity of carminic acid was linear with its concentration ranging from 1.0×10^-5 to 8.0×10^-5 mol/L,and the limit of detection(LOD)was 5.0×10^-6 mol/L.When the fluorescence method was applied to determine carminic acid in yogurts and Lactobacillus-containing beverages,the recoveries ranged from 96.6%to 110.5%,relative standard deviation(RSD)no more than 5.9%.This study provides an effective approach to rapidly and quantitatively detect carminic acid in foods.

关 键 词：胭脂虫红酸 荧光 增强 快速检测 

分 类 号：TS202.3[轻工技术与工程—食品科学] TQ617.1[轻工技术与工程—食品科学与工程]