The Fluorescence Immunochromatographic Strips for Natamycin and Its Application in Food Safety Detection  

作　　者：Wei ZHENG Yongfeng LIU Yafei XU Zhijun QIU Xinyue LI 

机构地区：[1]Beilun Customs,Ningbo 315000,China [2]Zhenhai District Agricultural Technology Extension Station,Ningbo 315000,China

出　　处：《Agricultural Biotechnology》2019年第6期104-105,共2页农业生物技术（英文版）

基　　金：Supported by Ningbo Entry-Exit Inspection and Quarantine Bureau(Ningbo Customs)Science and Technology Project(YK07-2017)

摘　　要：A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microspheres with a 2.5%(w/v) concentration. This labeled antibody was diluted for 10 times, and the diluted solution was dispensed into conjugate pad at the amount of 3 μl/cm. The concentrations of natamycin labeled BSA for test line and goat anti-mouse IgG for control line were 2.0 and 1 mg/ml, respectively, which performed best. With the best conditions, the limit of detection was 1 ng/ml, the linearity ranged from 2 to 100 ng/ml, the recovery was about 80% to 120%, and the CV was below 23%.A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microspheres with a 2.5%(w/v) concentration. This labeled antibody was diluted for 10 times, and the diluted solution was dispensed into conjugate pad at the amount of 3 μl/cm. The concentrations of natamycin labeled BSA for test line and goat anti-mouse IgG for control line were 2.0 and 1 mg/ml, respectively, which performed best. With the best conditions, the limit of detection was 1 ng/ml, the linearity ranged from 2 to 100 ng/ml, the recovery was about 80% to 120%, and the CV was below 23%.

关 键 词：Food safety detection NATAMYCIN Fluorescence immunochromatographic assay 

分 类 号：TS207.3[轻工技术与工程—食品科学]