Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila  

作　　者：Ting Cheng Yuanyuan Wang Jie Huang Xiao Chen Xiaolu Zhao Shan Gao Weibo Song 

机构地区：[1]Institute of Evolution and Marine Biodiversity,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266003,China [3]Department of Pathology,University of Michigan,Ann Arbor,MI 48109,USA [4]Department of Genetics and Development,Columbia University Medical Center,New York,NY 10032,USA [5]MOE Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China

出　　处：《Marine Life Science & Technology》2019年第1期4-14,共11页海洋生命科学与技术（英文）

基　　金：supported by Natural Science Foundation ofShandong Province (JQ201706);The Marine S&T Fund of ShandongProvince for Pilot National Laboratory for Marine Science and Technology(Qingdao) (2018SDKJ0406-2);Fundamental Research Fundsfor the Central Universities (201841005);the Blue Life BreakthroughProgram of LMBB of Qingdao National Laboratory for MarineScience and Technology (MS2018N004).

摘　　要：Epigenetic research focuses on heritable changes beyond the DNA sequence, which has led to a revolution in biologicalstudies and benefits in many other fields. The well-known model ciliate, Tetrahymena thermophila offers a unique system forepigenetic studies due to its nuclear dimorphism and special mode of sexual reproduction (conjugation), as well as abundantgenomic resources and genetic tools. In this paper, we summarize recent progress made by our research team and collaboratorsin understanding epigenetic mechanisms using Tetrahymena. This includes: (1) providing the first genome-wide basepair-resolution map of DNA N6-methyladenine (6mA) and revealed it as an integral part of the chromatin landscape;(2)dissecting the relative contribution of cis・ and trans- elements to nucleosome distribution by exploring the unique nucleardimorphism of Tetrahymena, (3) demonstrating the epigenetic controls of RNAi-dependent Polycomb repression pathwayson transposable elements, and (4) identifying a new histone monomethyltransferase, TXR1 (Tetrahymena Trithorax 1), thatfacilitates replication elongation through 让s substrate histone H3 lysine 27 monomethylation (H3K27mel).

关 键 词：EPIGENETICS TETRAHYMENA N6-methyladenine(6mA) NUCLEOSOME Transposable elements Histonemethyltransferase TXR1 

分 类 号：Q75[生物学—分子生物学]