响应面法优化莲花总黄酮超声辅助提取工艺及其抗氧化性的研究  

Study on the Optimum Extraction Technology and Antioxidant Activity of Total Flavonoids from Lotus

作　　者：贺文韬 林林 陈雨涔 陈浩 邓尚贵 HE Wen-tao;LIN Lin;CHEN Yu-cen(School of Food Science and Pharmaceutics of Zhejiang Ocean University,Zhoushan 316022;Zhoushan Academy of Agricultural and Forestry Sciences,Zhoushan 316000,China)

机构地区：[1]浙江海洋大学食品与医药学院,浙江舟山316022 [2]舟山市农林科学研究院,浙江舟山316000 [3]舟山市普陀农林局,浙江舟山316100

出　　处：《浙江海洋大学学报（自然科学版）》2019年第5期436-441,共6页Journal of Zhejiang Ocean University：Natural Science

基　　金：舟山市科技局科研项目(2018C13207)

摘　　要：为了探讨超声辅助乙醇浸提莲花总黄酮的最佳工艺条件及提取液的抗氧化性,在单因素实验的基础上,选取物料比、乙醇体积分数、浸提时间为自变量,黄酮提取率为响应值,设计3因素3水平的ox-Benhnken实验对莲花总黄酮的提取率进行优化,普鲁士兰法测定提取物的抗氧化性。结果表明,超声辅助提取莲花中总黄酮的最佳工艺条件为:物料比1:30(g·mL^-1)、乙醇体积分数70%、浸提时间29 min,提取次数2次,此条件下莲花总黄酮提取率最高为14.07%。与预测值13.92%.相近。此工艺条件下提取的莲花总黄酮其抗氧化性能随浓度升高而增大,低浓度时其抗氧化性比抗氧化剂BHT略弱,高浓度其氧化性远远大于BHT的抗氧化性。In order to explore the optimum technological conditions for ultrasonic assisted ethanol extraction of total flavonoids from lotus flower and the antioxidant activity of extracts,on the basis of single factor experiments,the ratio of materials,ethanol volume fraction and extraction time were selected as independent variables,and the extraction rate of flavonoids was the response value.The ox-Benhnken experiment with three factors and three levels was designed to optimize the extraction rate of total flavonoids from lotus flower.The extraction rate was determined by Prussian method.Antioxidant activity.The results showed that the optimum technological conditions for ultrasonic-assisted extraction of total flavonoids from lotus were as follows:material ratio 1:30(g·mL^-1),ethanol volume fraction 70%,extraction time 29 min and extraction times 2 times.Under this condition,the extraction rate of total flavonoids from lotus was 14.07%.It is close to the predicted value of 13.92%. Under this condition, the antioxidant activity of total flavonoids extracted from lotus flower increaseswith the increase of concentration. At low concentration, the antioxidant activity is slightly weaker than that ofantioxidant BHT, and at high concentration, the antioxidant activity is far greater than that of BHT.

关 键 词：莲花 响应面法 总黄酮 微波提取率 抗氧化性 

分 类 号：TS207.3[轻工技术与工程—食品科学]