碳源对耐低温哈尔滨不动杆菌HITLi 7~T氨氮代谢以及胞外分泌物的影响  

Effect of carbon sources on ammonia removal and extracellular polymeric substances secretion of Acinetobacter harbinensis HITLi 7~T

作　　者：郑泽嘉 李伟光 张淑梅[3] 马银鹏[3] 孟利强[3] ZHENG Zejia;LI Weiguang;ZHANG Shumei;MA Yinpeng;MENG Liqiang(School of Environment,Harbin Institute of Technology,Harbin 150090;State Key Laboratory of Urban Water Resources and Water Environment,Harbin 150090;Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010)

机构地区：[1]哈尔滨工业大学环境学院,哈尔滨150090 [2]城市水资源与水环境国家重点实验室,哈尔滨150090 [3]黑龙江省科学院微生物研究所,哈尔滨150010

出　　处：《环境科学学报》2019年第12期3950-3955,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.51578178,51608149)

摘　　要：碳源对微生物异养硝化至关重要,为了探究碳源对耐低温异养硝化菌-哈尔滨不动杆菌HITLi 7^T低温生长和氨氮代谢的影响,考察了乙酸钠、碳酸钠、淀粉、柠檬酸钠、蔗糖、葡萄糖、甘油、糊精、乳糖和麦芽糖10种碳源.结果表明,碳源种类对HITLi 7^T低温生长速率和胞外分泌物含量影响较大.乙酸钠是HITLi 7^T的最优碳源,低温下培养4 d后OD600能提高到0.146,产生的ATP也明显高于其他组,为5.124μmol·mg^-1·prot^-1;此时,总胞外分泌物量最多,为3.86 mg·L^-1,多糖占比也最高为41.7%;4 d氨氮平均降解速率最大,为0.042 mg·L^-1·h^-1;其次为葡萄糖和碳酸钠.同时考察了不同碳氮比的影响,结果表明HITLi 7^T以乙酸钠为碳源时,最佳C/N比为8,此时氨氮去除速率最高为0.216 mg·L^-1·h^-1,总EPS量为7.04 mg·L^-1,其中多糖占比为55.3%.In order to investigate the effect of carbon sources on the ammonia removal and the growth of heterotrophic nitrifier, Acinetobacter harbinensis HITLi 7^T, at low temperature, sodium acetate, sodium carbonate, starch, sodium citrate, sucrose, glucose, glycerol, dextrin and lactose were tested. Results found that the type of carbon source had a great influence on the growth and EPS secretion of HITLi 7^T at low temperature. Sodium acetate was the optimal carbon source and when served as the sole, after 4-day cultivation, the OD600 of HITLi 7^T reached 0.146, with the ATP at 5.124 μmol·mg^-1· prot^-1 and the average ammonia removal rate achieved 0.042 mg ·L^-1· h^-1. The performance of Glucose and sodium carbonate are next to sodium acetate. In addition, different type of carbon sources also had significant effects on extracellular polymeric substances(EPS) secretion of HITLi 7^T. When sodium acetate was the carbon source, the EPS amount of HITLi7^T was 3.86 mg· L^-1, with 41.7% of polysaccharide.It could be found that low C/N ratio was more suitable for HITLi 7^T. Ammonia removal rate reached maximum at 0.216 mg· L^-1 ·h^-1 as C/N was 8. At the same time, the EPS amount was 7.04 mg ·L^-1 with 55.3% polysaccharide.

关 键 词：低温 胞外分泌物 氨氮代谢 异养硝化菌HITLi 7^T 

分 类 号：X17[环境科学与工程—环境科学]