苏博美利奴羊胚胎期WNT2基因的组织表达及其生物信息学预测分析  

Tissue Expression and Bioinformatics Predictive Analysis of WNT2 Gene in Subo Merino Sheep during Embryonic Stage

作　　者：杨雪梅 范雪 田可川[2] 黄锡霞[1] 朱桦 阿布来提·苏来曼[1] 陈华峰 何军敏 赵冰茹[2] 徐新明[2] 付雪峰[2] 田月珍[2] 吴伟伟[2] 哈尼克孜·吐拉甫[2] 杜建文 YANG Xue-mei;FANG Xue;TIAN Ke-chuan;HUANG Xi-xia;ZHU Hua;Ablat Sulayman;CHEN Hua-feng;HE Jun-min;ZHAO Bing-ru;XU Xin-ming;FU Xue-feng;TIAN Yue-zhen;WU Wei-wei;Hanilazi Tulapu;DU Jian-wen(College of Animal Science,Xinjiang Agricultural University,Urumqi,830052,China;Research Institute of Animal Sciences,Xinjiang Academy of Animal Sciences,Urumqi,830011,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]新疆畜牧科学院畜牧研究所,乌鲁木齐830011

出　　处：《新疆农业科学》2019年第12期2320-2328,共9页Xinjiang Agricultural Sciences

基　　金：自治区重点实验室项目"新疆绒毛用羊遗传育种与繁殖"(XJYS1105);国家绒毛用羊现代产业技术体系建设项目(CAR-40)~~

摘　　要：【目的】检测WNT2基因在苏博美利奴羊胚胎期135 d不同组织中的表达水平,了解WNT2基因的分子结构和进化特征,从分子水平及进化特征上研究苏博美利奴羊的组织器官与WNT2基因表达之间的内在联系,为筛选细毛羊毛囊发育相关候选基因提供理论依据。【方法】采用qRT-PCR法研究苏博美利奴羊毛囊成熟时期WNT2基因在不同组织中的表达,并使用PROMO软件预测WNT2基因启动子区域序列的转录因子,并用Cytoscape_v3.5.1软件可视化,使用MEGA 7.0软件分析WNT2基因启动子区域序列的核苷酸组成成分及密码子的偏好性,并且利用绵羊、山羊、牛、人等9个物种的WNT2基因的DNA序列构建系统进化树。【结果】WNT2基因在皮肤组织中的表达量极显著高于心脏、肝脏、脾脏、肺脏、肾脏及肌肉组织。在绵羊WNT2基因启动子区域序列共预测出101个相关转录因子,4种碱基呈现均匀分布,CAG与UCU是使用相对较多的密码子,对WNT2基因的DNA序列系统进化树分析发现山羊和绵羊之间的进化关系比与其他哺乳动物进行比较时更为接近。【结论】建立了胚胎期135 d的苏博美利奴羊的不同组织中WNT2基因表达量的RT-PCR方法,并使用生物信息学软件对其分子结构和进化特征进行分析。【Objective】To detect the expression level of WNT2 gene in different tissues of Summerino sheep embryo stage and the molecular structure and evolution characteristics of WNT 2 gene in the hope of providing theoretical basis for screening candidate genes related to hair follicles development in this paper.【Methods】In this study,the expression of the WNT 2 gene in different tissues of the hair follicle of the Merino sheep was studied by qRT-PCR,and the transcription factor of the promoter region of the WNT 2 gene was predicted by using the PROMO software,and visualized by the software of Cytscape v3.5.1.The promoter region sequence of WNT 2 gene was analyzed by MEGA 7.0 software,and the preference of the codon was analyzed,and the phylogenetic tree was constructed using the DNA sequence of the WNT 2 gene of 9 species,such as sheep,goat,cattle and human.【Results】The results showed that the expression of WNT 2 gene in skin tissue was significantly higher than that in heart,liver,spleen,lung,kidney and muscle tissue.A total of 101 related transcription factors were predicted in the promoter region of sheep WNT 2 gene,and four bases were uniformly distributed.CAG and UCU used relatively many codon.The phylogenetic tree analysis of DNA sequence of WNT 2 gene showed that the evolutionary relationship between goat and sheep was closer than that of other mammals.【Conclusion】In this experiment,the RT-PCR method of WNT 2 gene expression in different tissues of the 135-day embryo stage of Subo Meino sheep was established and its molecular structure and evolution characteristics were analyzed by using bioinformatics software.The internal relationship between the expression of the tissue organ and the WNT 2 gene was discussed from the molecular level and the evolutionary character.

关 键 词：WNT2基因 表达量 转录因子 密码子 系统进化树 

分 类 号：S826[农业科学—畜牧学] S852.3[农业科学—畜牧兽医]