紫地榆多酚提取工艺优化及其体外抗氧化活性研究  

Extraction Technology of Polyphenol and in Vitro Antioxidant Activities from the Geranium strictipes R.Knuth

作　　者：罗廷顺 石桂兰 杨静 施边贵 蓝海 陈俊雅 LUO Ting-shun;SHI Gui-lan;YANG Jing;SHI Bian-gui;LAN Hai;CHEN Jun-ya(Dali Institute For Food and Drug Control,Dali Yunnan 671000,China;Dali Second Peoples Hospital,Dali Yunnan 671000,China;College of Pharmacy and Chemistry,Dali University,Dali yimnan 671000,China)

机构地区：[1]大理州食品药品检验所,云南大理671000 [2]大理州第二人民医院,云南大理671000 [3]大理大学药学与化学学院,云南大理671000

出　　处：《时珍国医国药》2019年第12期2853-2856,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81260512);大理大学创新训练基金项目(KYXI2018-08)。

摘　　要：目的优化紫地榆多酚提取工艺,并考察紫地榆多酚体外抗氧化活性方法采用超声法提取紫地榆多醐,在760nm处测定多酬的含量,并通过单因素试验和L9(3^3)正交设计试验对提取工艺进行优化以维生素C(Ve)为阳性对照,探讨紫地榆多酚对DPPH和ABTS4自由基的清除能力结果提取紫地榆多酚的最佳工艺条件为提取溶剂25%乙醇,提取时间30min,超声功率698W,提取紫地榆多盼的含量可达290.21 mg•g^1此条件下提取得到的紫地榆多酚清除DPPH•和ABTS+·的能力良好,尤其对ABTS+·清除能力最优,其IC50值分别为8.30,1.16 mg•L^-1.结论优化的提取工艺稳定、合理,紫地榆多酚有较好的抗氧化活性并呈现出良好的量效关系.Objective To optimize the extraction condition of polyphenol from the Geranium strictipes R.Knuth,and study its an-tioxidant activity in vitro.Methods The polyphenol were extracted by ultrasonic from the Geranium strictipes R.Knuth,the content of polyphenol was determined at 760 nm,and the extraction technology was optimized with single factor experiment and L9(3^3)orthogonal design.With the ascorbic acid as the contrast,its scavenging activities of DPPH and ABTS were discussed.Results The optimum condition of polyphenol extraction:extraction time was 30 min,extraction solvent was 25% ethanol solution,ultrasonic power was 698 W,and the content of polyphenol extracted was up to 290.21 mg•g^1.The polyphenol extracted from the Geranium strictipes R.Knuth under these conditions had good ability to scavenge several free radicals,especially ABTS+,the IC50 of DPPH and ABTS+were 8.30,1.16 mg•L^1.Conclusion The optimized extraction technology was stable and reasonable.The polyphenol of Geranium strictipes R.Knuth had better antioxidant activity and show a good dose-effect relationship.

关 键 词：紫地榆 多酚 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]