不同品种彩粒小麦苗麦绿素提取工艺优化及其含量比较  

Extraction Technology Optimization and Content Comparison of Barley Green from Different Varieties of Wheat

作　　者：杨文平 冯芳 郝教敏[3] 夏清[4] 靳明凯 杨珍平[4] 高志强[4] YANG Wen-ping;FENG Fang;HAO Jiao-min;XIA Qing;JIN Ming-kai;YANG Zhen-ping;GAO Zhi-qiang(College of Life Sciences,North China University of Science and Technology,Tangshan 063210,China;Tianjin University of Science&Technology,Tianjin 300222,China;College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,China;College of Agriculture,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]华北理工大学生命科学学院,河北唐山063210 [2]天津科技大学,天津300222 [3]山西农业大学食品科学与工程学院,山西太谷030801 [4]山西农业大学农学院,山西太谷030801

出　　处：《中国调味品》2020年第2期171-175,191,共6页China Condiment

基　　金：山西农谷建设科研专项(SXNGJSKYZX201701);山西省重点研发计划重点项目(201703D211001-02-02);国家现代农业小麦产业技术体系建设专项(CARS-03-01-24);山西省留学基金委员会重点资助项目(2015-重点4);山西省黄土高原特色作物优质高效生产协同创新中心(2016[5]);山西省“1331工程”重点实验室

摘　　要：以类黄酮、蛋白质含量和超氧化物歧化酶(Superoxide Dismutase,SOD)活性作为评价指标,试验采用水浸提法,通过单因素试验和L9(34)正交试验,考察了浸提温度、浸提时间和料液比对山农紫小麦、黑小麦031244、紫小麦204W17、黑小麦202W20 4个品种彩粒小麦苗麦绿素制备的影响,确定其提取的最佳工艺,并对其麦绿素含量进行比较。结果表明,紫小麦204W17苗麦绿素中类黄酮和蛋白质含量均最高,分别为40.21mg/100g和49.04%,其提取的最佳工艺条件为浸提温度25℃,浸提时间2.0h,浸提料液比1∶4;山农紫小麦苗麦绿素中SOD含量最高,为1865.3U/100g,其提取的最佳工艺条件为浸提温度30℃,浸提时间2.5h,浸提料液比1∶3。综合评价得出紫小麦204W17苗和山农紫小麦苗的麦绿素含量更高。The single factor experiment and L9(34)orthogonal experiment are conducted to investigate the effect of extracting temperature,extracting time and the ratio of solid to liquid on the preparation of barley green from four varieties of color-grained wheat seedlings including Shannong purple wheat,triticale 031244,purple wheat 204 W17 and triticale 202 W20 according to the content of flavonoids,protein and superoxide dismutase(SOD)by water extraction method,to determine the optimum extraction process of barley green and to compare its content among these varieties.The results show that the content of flavonoids and protein in barley green from purple wheat 204 W17 seedlings is the the highest of 40.21 mg/100 g and 49.04% respectively,and the optimal extracting conditions are concluded as follows:the extracting temperature is 25℃,the extracting time is 2.0 hand the ratio of solid to liquid is 1∶4.The content of SOD in barley green from Shannong purple wheat seedlings is the highest of 1865.3 U/100 g,and its optimal extracting conditions are concluded as follows:the extracting temperature is 30 ℃,the extracting time is 2.5 hand the solid-liquid ratio is 1∶3.Comprehensive evaluation shows that both purple wheat 204 W17 and Shannong purple wheat seedlings have higher-content barley green.

关 键 词：品种 彩粒小麦苗 麦绿素 提取 最佳工艺 

分 类 号：TS201.1[轻工技术与工程—食品科学]