阿魏酸对高糖诱导的人晶状体上皮细胞损伤的保护作用  

Effects of ferulic acid in reducing injury of human lens epithelial cells induced by high glucose

作　　者：朱德军[1] 王潇飞 薛中淇 扈晓雯[1] 夏明芳 冶敏 王力 邹文青[1] ZHU Dejun;WANG Xiaofei;XUE Zhongqi;HU Xiaowen;XIA Mingfang;YE Min;WANG Li;ZOU Wenqing(Department of Ophthalmology,Ningxia Eye Hospital,Ningxia Hui Autonomous Region People Hospital,Yinchuan 750002,Ningxia Hui Autonomous Region,China;Department of Pathology,North China University of Science and Technology Affiliated Hospital,Tangshan 063000,Hebei Province,China)

机构地区：[1]宁夏回族自治区人民医院宁夏眼科医院,西北民族大学第一附属医院,宁夏回族自治区银川市750002 [2]华北理工大学附属医院病理科,河北省唐山市063000

出　　处：《眼科新进展》2020年第2期115-119,共5页Recent Advances in Ophthalmology

基　　金：宁夏自然科学基金项目(编号:2018AA-C03176);宁夏致盲性眼病临床医学研究中心创新平台项目

摘　　要：目的探讨阿魏酸对高糖诱导的人晶状体上皮细胞(human lens epithelial cells,HLEC)损伤的保护作用。方法以HLEC为研究对象,将细胞随机分为对照组(HLEC+DMEM)、DMSO组(HLEC+DMEM+DMSO)、高糖组(HLEC+DMEM+30 mmol·L^-1高糖)、高糖+阿魏酸组(HLEC+DMEM+DMSO+30 mmo·L^-1高糖+阿魏酸)。采用CCK-8法检测各组细胞增殖情况;流式细胞术检测各组细胞凋亡;Q-PCR检测p53、Bcl-2、Bax mRNA表达水平;免疫细胞化学和Western blot法检测p53、Bcl-2、Bax蛋白表达水平。结果高糖抑制HLEC的细胞活性,不同浓度阿魏酸干预后,HLEC的细胞活性均明显升高,差异均具有统计学意义(均为P<0.05)。流式细胞术检测结果显示,高糖组HLEC凋亡率高于对照组(P<0.05),高糖+阿魏酸组细胞凋亡率低于高糖组(P<0.05);高糖组p53、Bax mRNA和蛋白表达水平均明显高于对照组,而Bcl-2 mRNA和蛋白表达水平均明显低于对照组(均为P<0.05);高糖+阿魏酸组p53、Bax mRNA和蛋白表达水平均明显低于高糖组,而Bcl-2 mRNA和蛋白表达水平均明显高于高糖组(均为P<0.05)。结论阿魏酸可能通过降低Bax和促进Bcl-2表达,进而减轻高糖诱导的HLEC损伤,从而达到防治糖尿病白内障发生的作用。Objective To investigate the effects of ferulic acid on the injury of human lens epithelial cells(HLEC)induced by high glucose.Methods HLEC were randomly divided into 4 groups:Control group(HLEC+DMEM),DMSO group(HLEC+DMEM+DMSO),high glucose group(HLEC+DMEM+30 mmol·L^-1 high glucose)and high glucose+ferulic acid group(HLEC+DMEM+DMSO+30 mmol·L^-1 high glucose+ferulic acid).Cell proliferation was determined by CCK-8 kit,cell apoptosis by flow cytometry,mRNA expression levels of p53,Bcl-2 and Bax by Q-PCR,and protein levels of p53,Bcl-2 and Bax by immunocytochemistry and Western blot.Results High glucose solution inhibited HLEC vitality,which obviously improved by intervention of different concentrations of ferulic acid(all P<0.05).Results of flow cytometry showed that the apoptosis rate of HLEC in the high glucose group was significantly higher than those in the control group and high glucose+ferulic acid group(all P<0.05).The mRNA and proteins levels of p53 and Bax in high glucose group were significantly higher than those in control group,but the mRNA and protein levels of Bcl-2 in high glucose group was significantly lower than those in control group(all P<0.05).The mRNA and protein levels of p53 and Bax in high glucose+ferulic acid group were significantly lower than that in high glucose group,but the mRNA and protein levels of Bcl-2 in high glucose+ferulic acid group were significantly higher than those in high glucose group(all P<0.05).Conclusion Ferulic acid may protect HLEC from injury induced by high glucose through decreasing Bax and promoting Bcl-2 expression,so as for prevention and treatment of diabetic cataract.

关 键 词：阿魏酸 晶状体上皮细胞 凋亡 P53 

分 类 号：R776[医药卫生—眼科]