人骨髓胎儿间充质干细胞在小鼠体内的分化  

In vivo differentiation of human fetal mesenchymal stem cells from bone marrow in mice

作　　者：朱慧 吴菲菲 王正梅 张昆龙 杨依 王亚云 Zhu Hui;Wu Feifei;Wang Zhengmei;Zhang Kunlong;Yang Yi;Wang Yayun(Department of Anatomy,Medical College of Yan’an University,Yan’an 716000;Preclinical Medical Teaching Experiment Center,Air Force Medical University,Xi’an 710032,China)

机构地区：[1]延安大学医学院解剖学教研室,延安716000 [2]空军军医大学基础医学院基础医学教学实验中心,西安710032

出　　处：《解剖学杂志》2020年第1期10-13,F0003,共5页Chinese Journal of Anatomy

基　　金：陕西省重点研发项目（2018JZ8003）;延安大学项目（YDYJG2017025）

摘　　要：目的:探讨人骨髓胎儿间充质干细胞(hfMSC)的特性及其在X染色体-连锁肌萎缩(mdx)小鼠体内分化的可能性。方法:从胎儿骨髓中分离扩增hfMSC,检测hfMSC Oct-4和Nanog-3的表达。经DIR标记后注射hfMSC到mdx小鼠腹股沟三角皮下,采用活体成像法观察小鼠离体后肢肌肉植入细胞的存活状态,观察是否出现畸胎瘤,并采用免疫荧光染色法检测dystrophin的表达。结果:从胎儿骨髓中分离到表达Oct-4和Nanog-3的hfMSC。在注射hfMSC的mdx小鼠离体后肢肌肉中检测到明显的荧光信号,并在肌肉组织中检测到人dystrophin的表达,而没有发现畸胎瘤的出现。结论:hfMSC表达多潜能抗原标志Oct-4和Nanog-3,hfMSC在mdx小鼠体内可以产生dystrophin,且不会形成畸胎瘤。Objective:To investigate the character of human fetal mesenchymal stem cell(hfMSC)and their feasibility of in vivo differentiation in mdx mice.Methods:hfMSC were isolated from fetal bone marrow and multipotent antigen markers Oct-4 and Nanog-3 were identified by immunostaining.hfMSC marked with DIR was injected into hypodermic inguinal triangle of mdx mice.Imaging system in vivo was used to detect the in vivo distribution of grafted hfMSCs.Presence or absence of teratoma was investigated and expression of human dystrophin was detected by immunofluorescence staining.Results:hfMSC expressing Oct-4 and Nanog-3 were isolated from fetal bone marrow.Significant fluorescence signals were detected in muscle of posterior limb of mdx mice with hypodermic injection of DIR-marked hfMSCs while human dystrophin expression was detected by immunofluorescence staining.However,no teratoma was found.Conclusion:hfMSC could express multipotent antigen markers Oct-4 and Nanog-3.hfMSC could express dystrophin in mdx mice but no teratoma is formed.

关 键 词：骨髓 胎儿间充质干细胞 多潜能抗原标志 移植 DYSTROPHIN 

分 类 号：R746.2[医药卫生—神经病学与精神病学]