人参皂苷Rg1、Rb1抗转基因斑马鱼模型的Aβ血管损害作用  

Effects of ginsenoside Rg1 and Rb1 against Aβ vascular injury in transgenic zebrafish models

作　　者：刘南海 黄晓峰[2] 符雪涛 王振凤[4] 薛寿儒[3] 杨凯[5] LIU Nan-hai;HUANG Xiao-feng;FU Xue-tao;WANG Zhen-feng;XUE Shou-ru;YANG Kai(Department of Neurology,the Second Affiliated Hospital of Gannan Medical University,Ganzhou 341600,Jiangxi,China)

机构地区：[1]赣南医学院第二附属医院神经内科,江西赣州341600 [2]赣南医学院护理学院,江西赣州341000 [3]苏州大学附属第一医院神经内科,江苏苏州215000 [4]赣南医学院研究生院,江西赣州341000 [5]赣南医学院药学院,江西赣州341000

出　　处：《广东医学》2020年第1期9-13,共5页Guangdong Medical Journal

基　　金：江西省教育厅科学技术研究项目(GJJ150960);赣南医学院科研项目(CP201407)

摘　　要：目的探究人参皂苷Rg1、Rb1对β-淀粉样蛋白(Aβ)导致转基因斑马鱼模型的血管损害作用的保护机制。方法选择正常健康转基因斑马鱼15尾,使用Aβ溶液诱导血管损伤转基因斑马鱼45尾,分为模型组、人参皂苷Rg1组和人参皂苷Rb1组各15尾。人参皂苷Rg1组和人参皂苷Rb1组分别使用20 mg/kg的Rg1、Rb1处理;检测各组转基因斑马鱼的血管长度和血管面积;使用ELISA检测各组转基因斑马鱼的血清中活性氧(ROS)、超氧物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力;使用Western blot检测各组斑马鱼转化生长因子β1(TGF-β1)、Smad2、Smad3及血管内皮生长因子(VEGF)表水平。结果与对照组比较,模型组血管长度、血管面积、SOD、GSH-Px水平和VEGF蛋白表达水平显著降低,ROS水平、TGF-β1、Smad2、Smad3蛋白表达水平显著升高,差异有统计学意义(P<0.05);相比模型组,人参皂苷Rg1组和人参皂苷Rb1组斑马鱼血管长度、血管面积、SOD、GSH-Px水平和VEGF蛋白表达水平显著升高,ROS水平、TGF-β1、Smad2、Smad3蛋白表达水平显著降低,差异有统计学意义(P<0.05)。结论人参皂苷Rg1、Rb1通过降低氧化应激及炎症反应并调节TGF-β1/Smads通路实现减轻Aβ对血管的损害。Objective To investigate the protective mechanism of ginsenoside Rg1 and Rb1 on vascular injury in transgenic zebrafish models induced by amyloid β protein(Aβ).Methods Fifteen healthy transgenic zebrafishes were enrolled, and 45 transgenic zebrafishes with vascular injury were induced by Aβ solution. The transgenic subjects were divided into model group, ginsenoside Rg1 group and ginsenoside Rb1 group(n=15). The ginsenoside Rg1 group and ginsenoside Rb1 group were treated with 20 mg/kg Rg1 and Rb1, respectively. The vascular length and area of transgenic zebrafishes in each group were detected. Elisa was performed to detect vitality of serum reactive oxygen species(ROS), superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px). WB was performed to detect expression levels of transforming growth factor β1(TGF-β1), Smad2, Smad3 and VEGF. Results Compared with control group, vascular length, vascular area, SOD, GSH-Px level and expression level of VEGF protein were significantly reduced in model group;while ROS level, and expression levels of TGF-β1, Smad2 and Smad3 were significantly increased(P<0.05). Compared with model group, vascular length, vascular area, SOD, GSH-Px level and expression level of VEGF protein were significantly increased in Rg1 group and ginsenoside Rb1 group;while ROS level, and expression levels of TGF-β1, Smad2 and Smad3 were significantly reduced(P<0.05). Conclusion Ginsenoside Rg1 and Rb1 can alleviate damage of Aβ to blood vessels by reducing oxidative stress and inflammatory response, and regulating TGF-β1/Smads pathway.

关 键 词：血管损伤 Β-淀粉样蛋白 人参皂苷 斑马鱼 

分 类 号：R741.02[医药卫生—神经病学与精神病学] R592[医药卫生—临床医学]