木聚糖酶原位协同水解预处理大米草的研究  

In-situ synergistic hydrolysis of pretreated Spartina by xylan enzymes

作　　者：杨旸 刘健 甘礼惠 龙敏南 YANG Yang;LIU Jian;GAN Li-hui;LONG Min-nan(College of Energy,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门大学能源学院

出　　处：《应用化工》2020年第1期74-77,84,共5页Applied Chemical Industry

基　　金：福建省海洋高新产业发展专项项目(闽海洋高新[2014]25)

摘　　要：用氨水与双氧水预处理大米草,与原料大米草一起进行球磨,用于木聚糖酶的协同酶解。结果表明,氨水结合双氧水预处理能有效脱除大米草中的木质素(63.81%),球磨处理能对纤维素结晶结构有所破坏,两者均可有效提高木聚糖酶的水解率。酶水解物主要成分为木糖(12.54%)、木二糖(40.38%)及少量阿拉伯糖(5.50%)。碱预处理结合球磨预处理,对木聚糖酶协同水解大米草产木寡糖具有明显的促进作用。The Spartina is pretreated with ammonia/hydrogen peroxide,and then mashes with Spartina,which was used for xylanse coenzyme hydrolysis.The results showed that ammonia/hydrogen peroxide pretreatment could effectively remove lignin from Spartina(63.81%),and ball milling treatment can destroy the crystal structure of cellulose.The two pretreatments both can effectively improve the hydrolysis efficiency of xylan enzymes.The main components of the enzymatic hydrolysate of ammonia/hydrogen peroxide combined with ball milling pretreated Spartina are xylose(12.54%),xylobiose(40.38%),and a small amount of arabinose(5.50%).The ammonia/hydrogen peroxide pretreatment combined with ball milling pretreatment can significantly enhance the synergistic hydrolysis efficiency of xylan enzymes to produce xylooligosaccharides from Spartina.

关 键 词：碱预处理 球磨预处理 协同水解 木糖 木寡糖 

分 类 号：TQ150[化学工程—电化学工业]