绿茶多酚联合甲钴胺对周围神经损伤修复作用的动物实验研究  

Experimental study on the effect of green tea polyphenols combined with Mecobalamin on the repair of peripheral nerve injury

作　　者：杨荣源 陈进鸿 李洪瀚 yang Rongyuan;Chen Jinhong;Li Honghan(Department of Orthopedics,Zhangzhou Hospital Affiliated to Fujian Medical University,Fujian 363000,China)

机构地区：[1]福建医科大学附属漳州市医院骨科,福建363000

出　　处：《中华手外科杂志》2020年第1期61-65,共5页Chinese Journal of Hand Surgery

基　　金：手功能重建卫生部重点实验室及上海市周围神经显微外科重点实验室开放课题(17DZ2270500)。

摘　　要：目的探讨绿茶多酚联合甲钻胺对坐骨神经损伤修复后神经再生的影响。方法将60只成年Wistar大鼠随机分为两组,实验组和对照组,每组30只,均行右侧坐骨神经离断后神经外膜端端缝合,制作周围神经损伤修复模型。另取6只Wistar大鼠作为空白对照组。实验组以绿茶多酚50 mg/(kg・d)、甲钻胺50 ug(kg・d)灌胃法给药,对照组仅以甲钻胺50 ug/(kg・d)灌胃法给药,连续给药2周。随后观察坐骨神经修复后的一般情况,神经电生理恢复情况,小腿三头肌湿重恢复率,有髓神经纤维计数及组织病理形态学差异。结果术后2、4、6周神经平均传导速度和有髓神经纤维计数实验组均明显高于对照组,差异有统计学意义(P<O.05);而术后8周两组比较差异无统计学意义(0.05);术后12周实验组、对照组和空白对照组比较差异均无统计学意义(p>0.05)。术后4、8、12周小腿三头肌湿重恢复率实验组明显高于对照组,差异有统计学意义(P<0.05)。实验组再生的有髓神经纤维分布均匀,排列相对整齐,密度较高,结缔组织增生较少。结论绿茶多酚可能在促进周围神经再生方面发挥较为重要的作用。Objective To investigate the effect of green tea polyphenols combined with Mecobalamin on nerve regeneration after sciatic nerve injury and repair.Methods Sixty adult Wistar rats were randomly divided into two groups,the experimental group and the control group,30 rats in each group.Another 6 Wistar rats were taken as the blank control group.The experimental group was given green tea polyphenols 50 mg/(kg•d)and Mecobalamin 50ug/(kg•d)by gavage,while the control group was given Mecobalamin 50 ug/(kg•d)by gavage for 2 weeks.Then the general condition,electrophysiological recovery,wet weight recovery rate of triceps surae muscle,the number of myelinated nerve fibers and the difference of histopathology were observed.Results The average nerve conduction velocity and myelinated nerve fiber count in the experimental group were significantly higher than those in the control group at the 2nd,4th and 6th week after operation(P<0.05),but there was no significant difference between the two groups at the 8th week after operation(P>0.05),and there was no significant difference between the experimental group,the control group and the blank control group at the 12th week after operation(P>0.05).The recovery rate of wet weight of triceps surae muscle in experimental group was significantly higher than that in control group(P<0.05).In the experimental group,the regenerated myelinated nerve fibers were evenly distributed,arranged relatively orderly,with high density and less hyperplasia of connective tissue.Conclusion The green tea polyphenols may play an important role in promoting peripheral nerve regeneration.

关 键 词：周围神经 神经再生 绿茶多酚 甲钻胺 

分 类 号：R74[医药卫生—神经病学与精神病学]