不同剂量咖啡对非酒精性脂肪性肝病模型大鼠脂质代谢的影响  

Effects of different doses of coffee on lipid metabolism in rats with nonalcoholic fatty liver disease

作　　者：马欢[1] 赵新华[1] 张丽丽[1] MA Huan;ZHAO Xinhua;ZHANG Lili(Department of Gastroenterology,Mianyang Central Hospital,Mianyang 621000,Sichuan China)

机构地区：[1]绵阳市中心医院消化内科

出　　处：《西部医学》2020年第1期38-42,共5页Medical Journal of West China

基　　金：四川省卫生和计划生育委员会科研课题(18PJ395)

摘　　要：目的探讨不同剂量咖啡对非酒精性脂肪性肝病模型大鼠脂质代谢的影响。方法选取80只6周龄SPF级SD大鼠,体重(180±20)g,随机分为空白组、模型组、高剂量咖啡组及低剂量咖啡组4组,每组各20只。空白组予以普通饲料喂养,其他3组采用高脂饮食喂养8周,建立非酒精性脂肪性肝病大鼠模型。高、低剂量咖啡组每天分别予以60、20mg/kg咖啡因灌胃,空白组与模型组予以生理盐水灌胃,持续8周。8周后,检测大鼠体重及肝指数变化;采用全自动生化分析仪测定大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)、r-谷氨酸转移酶(GGT)、甘油三酯(TG)、总胆固醇(TC)含量变化、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C);使用HE染色观察肝脏的病理形态变化。结果与空白组比较,模型组大鼠体重、肝指数、ALT、AST、GGT、TG、TC和LDL-C含量显著升高,HDL-C含量显著降低(P均<0.05)。与模型组比较,不同剂量咖啡组处理的大鼠肝指数、ALT、AST、GGT、TG、TC和LDL-C含量显著降低;且高剂量咖啡组上述指标显著低于低剂量咖啡组(P均<0.05)。与模型组比较,不同剂量咖啡组的HDL-C明显升高,且高剂量咖啡组明显高于低剂量咖啡组(P均<0.05)。模型组大鼠肝脏包膜紧张度高,边缘圆顿;使用咖啡处理后的大鼠肝脏边缘锐利。模型组大鼠肝组织弥漫小泡性肝细胞脂肪变,肝窦明显因水肿而狭窄,而使用咖啡处理后大鼠肝组织脂肪浸润现象显著减轻,肝细胞胞质均匀无水肿。结论咖啡可有效缓解大鼠脂肪肝的发展,通过调节脂质代谢降低肝脏内炎症反应,且咖啡调节脂质代谢在一定浓度范围内呈浓度依赖性。咖啡可使肝脏质感柔嫩、有弹性且边缘锐利、整齐。Objective To investigate the effect of different doses of coffee on lipid metabolism in rats with nonalcoholic fatty liver disease.Methods 80 6 week old SPF grade SD rats weighing(180 ± 20) g were randomly divided into blank group, model group, high-dose coffee group and low-dose coffee group. The rats in the blank group were fed with normal diet and the rats in the other three groups were fed with high-fat diet for 8 weeks. The high and low dose coffee group were given 60 and 20 mg/kg caffeine per day, and the blank group and the model group were given normal saline for 8 weeks. After 8 weeks, the body weight and liver index of rats were measured. The changes of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), R glutamate transferase(GGT), triglyceride(TG), total cholesterol(TC), high-density lipoprotein cholesterol(HDL C) and low-density lipoprotein cholesterol(LDL C) were measured by automatic biochemical analyzer. HE staining was used to observe the pathological changes of liver. ResultsCompared with the blank group, the body weight, liver index, alt, AST, GGT, TG, TC and LDL C contents of the model group increased significantly, while the HDL C content decreased significantly(P<0.05). Compared with the model group, the liver index, alt, AST, GGT, TG, TC and LDL C contents of the rats treated with different doses of coffee decreased significantly, and the above indexes of the high-dose coffee group were significantly lower than those of the low-dose Coffee Group(P<0.05). Compared with the model group, the HDL C of different dose coffee group was significantly higher, and the HDL C of high dose coffee group was significantly higher than that of low dose Coffee Group(P<0.05). In the model group, the tension of liver capsule was high and the margin was round. The edge of the liver of the rats treated with coffee was sharp. In the model group, the fatty change of hepatocytes was diffuse and vesicular, and the hepatic sinuses were narrowed obviously due to edema. However, the fatty infiltrati

关 键 词：非酒精性脂肪性肝病 咖啡 脂质代谢 HE染色 炎症反应 

分 类 号：R575.5[医药卫生—消化系统]